SC35 Antibody (SC-35)

Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated by SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:100. Lane 2: Antibody dilution 1:250. Lane 3: Antibody dilution 1:500. Lane 4: Antibody dilution 1:1000.

Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - Gadd45a localization in nuclear speckles is RNase sensitive Immunofluorescence confocal microscopy of detergent-extracted RKO cells. Cells were transfected with N-EGFP-Gadd45a and stained with antibodies against SC35and p68 . Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014500) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing Factor Clone: SC-35 diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, FITC-conjugate.

Product Discontinued

SC35 Antibody (SC-35) Summary

• Immunogen: Partially purified mammalian spliceosome.
• Epitope: This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35.
• Localization: Nuclear
• Marker: Nuclear Speckle Marker
• Specificity: Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRSF2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10 - 1:500
  • ELISA Indirect
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30015619). This SC35 Antibody (SC-35) is validated for IHC-Fr and IHC-Free Floating from a verified customer review. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification. IHC-P: See Nie et al 2002 paper where it appears no antigen retrieval is necessary. Block in normal serum 10% in TBS (pH 7.6) for 30min then incubate antibody overnight at 4C. Use ABC amplification- DAB detection. WB: Detects a doublet of approximately 35 kDa. *Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31600344).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for SC35 Antibody (SC-35)

• PR264
• SC35
• SC-35
• serine/arginine-rich splicing factor 2
• SFRS2
• SFRS2A
• splicing component, 35 kDa
• splicing factor SC35
• splicing factor, arginine/serine-rich 2
• SR splicing factor 2
• SRp30b

Background

SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SC35 Antibody (NB100-1774)(8)

Publications using NB100-1774

• Lehman BJ, Lopez-Diaz FJ, Santisakultarm TP, et al. Dynamic regulation of CTCF stability and sub-nuclear localization in response to stress PLoS genetics 2021-01-01 [PMID: 33411704] (WB, Human)
• Wu N, Nguyen XN, Wang L et al. The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation PLoS Pathog. 2019-10-01 [PMID: 31600344] (KO, Mouse, Human)
• Malik AM, Miguez RA, Li X et al. Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization Elife. 2018-07-16 [PMID: 30015619] (IHC-P, Rat)
• Grindheim AK, Hollas H, Raddum AM et al. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of Annexin A2. J. Cell. Sci. 2015-12-07 [PMID: 26644180] (ICC/IF, Rat)
• Sytnikova YA, Kubarenko AV, Schafer A et al. Gadd45a is an RNA binding protein and is localized in nuclear speckles. PLoS One;6(1). 2011-01-07 [PMID: 21249130]
• Nie GY et al. A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women rhesus monkeys. Reproduction 124:209-17. 2002-01-01 [PMID: 12141933]
• Fu XD Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343:437-41 (1990). [PMID: 2137203]
• Petri et al. Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2C{gamma} gover. J. Cell Bio. 179:451. 2007-01-01 [PMID: 1833187]

Reviews for SC35 Antibody (NB100-1774) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Rat.

reviewed by: Soledad Barez IHC-Free Floating sections Rat 11/25/2020

Summary

• Application: Other
• Sample Tested: Brain free floating slices,free-floating slices rat brain
• Species: Rat
• Lot: 94053

Comments

• Comments: Citrate Buffer Antigen Retrieval. 1:100 dilution, overnight incubation 4°.

reviewed by: Soledad Barez IHC-Fr Rat 11/25/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Fresh frozen rat brain
• Species: Rat
• Lot: 94053

Comments

• Comments: 1:100 dilution, overnight incubation at 4°C.
  No need for antigen retrieval.

Bioinformatics

• Gene Symbol: SRSF2
• Entrez:
  • 494445(Rat)