Sca-1/Ly6 Antibody (177228) [Unconjugated]

Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 177228
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Sca-1/Ly6 Antibody (177228) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
  Leu27-Gly119
  Accession # CAA28351
• Specificity: Detects mouse Sca‑1/Ly6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody (177228) [Unconjugated]

• Ly6
• Ly-6
• Ly-6A.2
• Ly-6A/E
• Ly-6E.1
• lymphocyte antigen 6 complex, locus A
• lymphocyte antigen 6A-2/6E-1
• Sca1
• Sca-1
• Stem cell antigen 1
• TAP
• T-cell-activating protein

Background

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
2. Spangrude, G.I. et al. (1988) Science 241:58.
3. Spangrude, G.I. et al. (1993) Blood 82:3327.
4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
6. Yamamoto, Y. et al. (1996, Blood 88:445.
7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sca-1/Ly6 Antibody (MAB1226)(12)

Publications using MAB1226

• Liu, Y;Yin, M;Mao, X;Wu, S;Wei, S;Heng, S;Yang, Y;Huang, J;Guo, Z;Li, C;Ji, C;Hu, L;Liu, W;Zhang, LJ; Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics eLife 2024-08-30 [PMID: 39213029] (Immunohistochemistry, Mouse)
• Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
• Hosaka K, Wang C, Zhang S et al. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors Cancer communications (London, England) 4/29/2023 [PMID: 37120719]
• Hosaka K, Wang C, Zhang S et al. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors Cancer communications (London, England) 2023-04-29 [PMID: 37120719]
• Tan GK, Pryce BA, Stabio A et al. Tgfbeta signaling is critical for maintenance of the tendon cell fate Elife 1/22/2020 [PMID: 31961320]
• Xiying Fan, Dongmei Wang, Jeremy Evan Burgmaier, Yudong Teng, Rose-Anne Romano, Satrajit Sinha et al. Single Cell and Open Chromatin Analysis Reveals Molecular Origin of Epidermal Cells of the Skin Developmental Cell 10/1/2018 [PMID: 30220568]
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)
• Hibino N, Villalona G, Pietris N Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel. FASEB J., 2011-05-12;25(8):2731-9. 2011-05-12 [PMID: 21566209] (IHC-P, Mouse)
• Eugenia C. Pacheco-Pinedo, Amy C. Durham, Kathleen M. Stewart, Ashley M. Goss, Min Min Lu, Francesco J. DeMayo et al. Wnt/ beta -catenin signaling accelerates mouse lung tumorigenesis by imposing an embryonic distal progenitor phenotype on lung epithelium Journal of Clinical Investigation 5/2/2011 [PMID: 21490395]
• Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M BCL6 is critical for the development of a diverse primary B cell repertoire. J. Exp. Med., 2010-05-24;207(6):1209-21. 2010-05-24 [PMID: 20498019] (Flow Cytometry, Mouse)
• Noh MS, Jun BH, Kim S, Kang H, Woo MA, Minai-Tehrani A, Kim JE, Kim J, Park J, Lim HT, Park SC, Hyeon T, Kim YK, Jeong DH, Lee YS, Cho MH Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice. Biomaterials, 2009-05-02;30(23):3915-25. 2009-05-02 [PMID: 19411103] (IHC, Mouse)
• Hegab AE, Kubo H, Yamaya M, Asada M, He M, Fujino N, Mizuno S, Nakamura T Intranasal HGF administration ameliorates the physiologic and morphologic changes in lung emphysema. Mol. Ther., 2008-06-17;16(8):1417-26. 2008-06-17 [PMID: 18560414] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: Ly6a
• Uniprot:
  • CAA28351()