Secondary Antibody Sampler- Goat anti-mouse and Goat anti-rabbit IgG Antibody Pack

Product Discontinued

Secondary Antibody Sampler- Goat anti-mouse and Goat anti-rabbit IgG Antibody Pack Summary

• Specificity: This kit provides six different HiLyte Fluor(TM) fluorescent secondary antibodies, which are ideal for optimizing your immunofluorescence-staining experiment. Each antibody is sufficient for staining 4-40 samples in size of 10 x 10 mm^2.
• Kit Type: Antibody Labeling Kit
• Isotype: IgG

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: All fluorescent secondary antibodies are supplied as 0.5 mg/mL in 0.01 mM phosphate, 150 mM NaCl, pH

Notes

1 vial each of: NB710-94932, NB710-94933, NB710-94934, NB710-94935, NB710-94936 and NB710-94937.

Background

Fluorescent dye conjugated secondary antibodies have been widely used in immunofluorescence staining, fluorescence activated cell sorting, in situ hybridization and other fluorescence-based biological applications. The serial HiLyte Fluor(TM) fluorophores have premium quality with brighter fluorescence and better photostability compared to FITC, TAMRA and Cy5 dyes. The HiLyte Fluor(TM) fluorescent secondary antibodies have been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. The actual dye/IgG ratio or degree of substitution (DOS) is labeled on each vial. Every lot has been validated with immunofluorescence staining to guarantee the best performance. The goat anti-rabbit IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein. While the goat anti-mouse IgG is highly adsorbed with minimal cross-reaction to human, bovine, horse, rabbit, and swine serum proteins. These secondary antibodies are ideal for multi-fluorescence staining in which several primary antibodies from different species are involved. All fluorescent secondary antibodies are also available to be purchased individually.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.