Serotonin Antibody (5HT-H209) - Unpurified

Immunohistochemistry: Serotonin Antibody (5HT-H209) - Unpurified [NB120-16007] - Expression of Serotonin, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain. Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (um). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/10614) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Serotonin Antibody (5HT-H209) [NB120-16007] - FFPE rhesus macaque proximal colon. Image from verified customer review.

Immunohistochemistry-Paraffin: Serotonin Antibody (5HT-H209) [NB120-16007] - Swine duodenum tissue stained with Serotonin antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Serotonin Antibody (5HT-H209) [NB120-16007] - Formalin-fixed, paraffin-embedded human appendix stained with Serotonin (NB120-16007) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of serotonin-secreting cells.

Product Discontinued

Serotonin Antibody (5HT-H209) - Unpurified Summary

• Immunogen: 5-Hydroxytryptamine hydrochloride (3-(2-aminoethyl)-5-hydroxyindole;serotonin).
• Localization: Cytoplasmic
• Specificity: Reacts with serotonin in a broad range of normal, hyperplastic and neoplastic tissues. Serotonin is also called 5-hydroxytryptamine. The antibody is of particular importance in identifying primary and metastatic carcinoid tumors expressing serotonin. NB120-16007 may be a useful tool for characterization of carcinoids. In a study of 48 cases of adenomas and carcinoid tumours of the middle ear, the labelled tumour cells in 12 cases (25%).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: Proteinase K antigen retrieval is recommended for FFPE tissues.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 27183534). Mouse reactivity reported in scientific literature (PMID: 27447971). Porcine, Rhesus macaque reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Cell culture supernatant dialysed against 0.05 mol/L Tris/HCl (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Serotonin Antibody (5HT-H209) - Unpurified

• 5-HT
• 5-hydroxytryptamne

Background

Serotonin-positive cells are present in gastric, duodenal, jejunal, ileal, colonic, and appendiceal mucosae. Positive cells are sparse and usually occur in the lower half of the gastric and intestinal mucosae. In the appendix, in addition to the mucosal serotonin-containing cells, there are single or small clusters of serotonin-positive cells in the sub-epithelial region of the lamina propria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serotonin Antibody (NB120-16007)(21)

Publications using NB120-16007

• Wang H, Zhao X, Cui X Et al. A Pilot Study of Clinical Evaluation and Formation Mechanism of Irritable Bowel Syndrome-like Symptoms in Inflammatory Bowel Disease Patients in Remission Journal of neurogastroenterology and motility 2021-10-30 [PMID: 34642282] (IF/IHC, Human)
• Dioguardi Burgio M, Cros J, Panvini N et al. Serotonin immunoreactive pancreatic neuroendocrine neoplasm associated with main pancreatic duct dilation: a recognizable entity with excellent long-term outcome European radiology 2021-05-11 [PMID: 33977308]
• Andersen DB, Grunddal KV, Pedersen J, et al. Using a Reporter Mouse to Map Known and Novel Sites of GLP-1 Receptor Expression in Peripheral Tissues of Male Mice Endocrinology 2021-03-01 [PMID: 33508122] (IHC-Fr, Mouse)
• Bhowmick S, Abdul-Muneer PM PTEN Blocking Stimulates Corticospinal and Raphespinal Axonal Regeneration and Promotes Functional Recovery After Spinal Cord Injury Journal of neuropathology and experimental neurology 2020-12-26 [PMID: 33367790]
• Wang Y, Sims CE, Allbritton NL Enterochromaffin Cell-Enriched Monolayer Platform for Assaying Serotonin Release from Human Primary Intestinal Cells Anal. Chem. 2020-09-01 [PMID: 32819098]
• Sugisawa E, Takayama Y, Takemura N et al. RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis Cell 2020-07-07 [PMID: 32640190] (IHC-P, Mouse)
• Negi S, Bolt CC, Zhang H, Stubbs L. An extended regulatory landscape drives Tbx18 activity in a variety of prostate-associated cell lineages Dev. Biol. 2018-12-27 [PMID: 30594504] (IHC-P, Mouse)
• Jung K, Miyazaki A, Hu H, Saif L. Susceptibility of porcine IPEC-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PDCoV) and serum cytokine responses of gnotobiotic pigs to acute infection with IPEC-J2 cell culture-passaged PDCoV Vet Microbiol. [PMID: 29981708] (ICC/IF, Porcine)
• Kluger N, Jokinen M, Lintulahti A et al. Gastrointestinal immunity against tryptophan hydroxylase-1, aromatic L-amino-acid decarboxylase, AIE-75, villin and Paneth cells in APECED. Clin. Immunol. 2015-03-21 [PMID: 25805658] (IHC-P, Human)
• Talchai C, Xuan S, Kitamura T et al. Generation of functional insulin-producing cells in the gut by Foxo1 ablation Nat Genet 2012-03-11 [PMID: 22406641] (IF/IHC, Mouse)
• Kluger N. et al. New insights in Autoimmune Polyendocrine Syndromes 1 and 2 Thesis. 2015-01-01 (IHC-P, Human)
• Omari S, Roded A, Eisenberg M et al. Mast cell secretory granule fusion with amphisomes coordinates their homotypic fusion and release of exosomes. Cell reports 2024-07-09 [PMID: 38985670]
• Rui Deng, Suyun Yu, Xingqiu Ruan, Huan Liu, Gangfan Zong, Peng Cheng, Ruizhi Tao, Wenxing Chen, Aiyun Wang, Yang Zhao, Zhonghong Wei, Yin Lu Capsaicin orchestrates metastasis in gastric cancer via modulating expression of TRPV1 channels and driving gut microbiota disorder Cell Communication and Signaling : CCS 2023-12-21 [PMID: 38129926]
• Bacon RL, Taylor L, Gray SB, Hodo CL Analysis of cell populations in the normal rhesus macaque (Macaca mulatta) lower intestinal tract and diagnostic thresholds for chronic enterocolitis Veterinary pathology 2023-10-11 [PMID: 37818978] (IHC-P)
• Renga G, D'Onofrio F, Pariano M et al. Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia Nature communications 2023-09-16 [PMID: 37717018]
• McKimpson WM, Spiegel S, Mukhanova M et al. Calorie Restriction activates a gastric Notch-FOXO1 pathway to expand Ghrelin cells bioRxiv : the preprint server for biology 2023-03-07 [PMID: 36945500]
• Rafeeqi TA, Diyaolu M, Thomas AL et al. Generation of Porcine Ileum Through Spring-Mediated Mechanical Distraction The Journal of surgical research 2022-08-26 [PMID: 36037614]
• Jung K, Miyazaki A, Saif LJ. Immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (Gn) pigs infected with porcine epidemic diarrhea virus (PEDV) and serum cytokine respo Res. Vet. Sci. 2018-06-12 [PMID: 29909130] (IF/IHC, Porcine)
• Gwynne WD, Hallett RM, Girgis-Gabardo A et al. Serotonergic system antagonists target breast tumor initiating cells and synergize with chemotherapy to shrink human breast tumor xenografts. Oncotarget 2017-05-09 [PMID: 28404880] (IHC-P, Mouse)
• Hallett RM, Girgis-Gabardo A, Gwynne WD et al. Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer. Oncotarget. 2016-08-16 [PMID: 27447971] (IF/IHC, ICC/IF, Mouse)
• Delgermurun D, Yamaguchi S, Ichii O et al. Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2016-05-13 [PMID: 27183534] (IHC-P, Chicken)
  
  Details:
  5-HT (Serotonin) antibody used for IHC-P on chicken aorta section (Fig. 4).

Reviews for Serotonin Antibody (NB120-16007) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Other, Swine.

reviewed by: Verified Customer IHC-P Swine 11/23/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: duodenum
• Species: Swine
• Lot: 20010335

reviewed by: Steven Laing IHC-P Other 07/18/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Formalin-fixed (72 hours) paraffin-embedded proximal colon
• Species: Other
• Lot: 20010335

FAQs for Serotonin Antibody (NB120-16007). (Showing 1 - 4 of 4 FAQs).

1. What was the immunogenic carrier protein for this serotonin antibody?
   • The immunogen for NB120-16007 was not conjugated to a carrier protein.
2. Does the anti-serotonin antibody work with serotonin itself?
   • This antibody is one of our best sellers and has been widely used for labeling cells expressing serotonin, especially the carcinoid tumors.
3. I need to know the molar concentration of this antibody.
   • The concentration for NB120-16007 (Serotonin Antibody/5HT-H209) Lot# 20010335 is 11 ug/ml. We normally regard the size for an IgG molecule as ~160 kDa. Maybe this information is helpful for you to convert it into the molar concentration.
4. Could you please provide us the suggested dilution of NB120-16007 for IHC-Fr on mouse sample?
   • This antibody was validated for IHC with frozen tissue samples based on citation in the following publication: NCBI site The authors of this article stained frozen sections of guinea pig pancreas with the antibody. They used NB120-16007 at a dilution of 1:200, with a long incubation (24-48 hours) at 4C.

Bioinformatics

• Entrez:
  • 15563(Mouse)
  • 25689(Rat)