Serotonin Antibody (YC5/45) - BSA Free

Immunohistochemistry-Paraffin: Serotonin Antibody (YC5/45) [NB100-65037] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Serotonin antibody (clone YC5/45). The signal was developed using HRP-DAB method and the nuclei were counterstained using hematoxylin. This antibody generated a specific strong staining in serotonin positive neurons.

• Reactivity: All
• Applications: ICC/IF, IHC, IHC
• Clone: YC5/45
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Serotonin Antibody (YC5/45) - BSA Free Summary

• Immunogen: Serotonin conjugated to Bovine Serum Albumin.
• Specificity: NB100-65037 recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin. Serotonin (also known as 5-hydroxytryptamine or 5-HT) is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry Whole-Mount reported in scientific literature (PMID 7781904)
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500

Reactivity Notes

This antibody detects serotonin in multiple species including human, mouse, rat, and other vertebrate and invertebrate animals.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Serotonin Antibody (YC5/45) - BSA Free

• 5-HT
• 5-hydroxytryptamne

Background

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20um) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. Useful for the diagnosis of carcinoid tumors and the investigation of the effects of drugs on the neurological system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serotonin Antibody (NB100-65037)(12)

Publications using NB100-65037

• Osborn P A Role for Serotonin in the Developing Xenopus Tadpole Visual System Thesis 2023-01-01 (IHC, Xenopus)
• Dunn GA The Role of Maternal Obesity and Consumption of a Western-Style Diet on Offspring Brain Development and Behavior via an Inflammatory Mechanism Thesis 2023-01-01 (IHC, Macaque)
  
  Details:
  Macaca fuscata, Japanese macaque
• Zelenika D Adams E Humm S et al. The role of CD4+ T-cell subsets in determining transplantation rejection or tolerance. Immunol Rev. [PMID: 11722632]
• Gil-Loyzaga P Bartolome V Vicente-Torres A Carricondo F. Serotonergic innervation of the organ of Corti. Acta Otolaryngol. [PMID: 11603756]
• Portela-Gomes GM Lukinius A Grimelius L. Synaptic vesicle protein 2, A new neuroendocrine cell marker. Am J Pathol. [PMID: 11021834]
• Pyza E Meinertzhagen IA. Neurotransmitters regulate rhythmic size changes amongst cells in the fly's optic lobe. J Comp Physiol A. [PMID: 8568723]
• Mukhopadhyay M Campos AR. The larval optic nerve is required for the development of an identified serotonergic arborization in Drosophila melanogaster. Dev Biol. [PMID: 7781904] (IHC-WhMt)
• Consolazione A Milstein C Wright B Cuello AC. Immunocytochemical detection of serotonin with monoclonal antibodies. J Histochem Cytochem. [PMID: 7033368] (IF/IHC)
• Milstein C Wright B Cuello AC. The discrepancy between the cross-reactivity of a monoclonal antibody to serotonin and its immunohistochemical specificity. Mol Immunol. [PMID: 6190077]
• Wells CA Taylor SM Cuello AC. Argentaffin and argyrophil reactions and serotonin content of endocrine tumours. J Clin Pathol. [PMID: 2578484] (IHC-P)
• Buc-Caron MH Launay JM Lamblin D Kellermann O. Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma. Proc Natl Acad Sci U S A. [PMID: 2155426]
• Solcia E Capella C Fiocca R et al. Gastric argyrophil carcinoidosis in patients with Zollinger-Ellison syndrome due to type 1 multiple endocrine neoplasia. A newly recognized association. Am J Surg Pathol. [PMID: 1970928] (IHC-P)