sFRP-1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

sFRP-1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human sFRP-1
  Ser32-Lys314
  Accession # AAB70793
• Specificity: Detects human sFRP-1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse sFRP-2, recombinant human (rh) sFRP-3, and rhsFRP-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SFRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for sFRP-1 Antibody [Biotin]

• FRP1
• FRP-1FrzA
• FRPSARP-2
• FrzA
• SARP-2
• SARP2sFRP-1
• Secreted apoptosis-related protein 2
• secreted frizzled-related protein 1
• sFRP1
• sFRP-1

Background

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are approximately 30 - 35 kDa in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1).

sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2, 5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).

1. Jones, S. et al. (2002) Bioessays 24:811.
2. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
3. Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.
4. Finch, P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
5. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
6. Ugolini, F. et al. (2001) Oncogene 20:5810.
7. Ko, J. et al. (2002) Exp. Cell. Res. 280:280.
8. Roth, W. et al. (2000) Oncogene 19:4210.
9. Fukuhara, K, et al. (2002) J. Clin. Endocr. Metab. 87:1729.
10. Dufourcq, P. et al. (2002) Circulation 106: 3097.
11. Yoshino, K. et al. (2001) Mech. Dev. 102:45.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFRP1
• Uniprot:
  • AAB70793()