SGK3 Antibody

Immunohistochemistry-Paraffin: SGK3 Antibody [NLS7240] - Anti-SGK3 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: SGK3 Antibody [NLS7240] - Analysis of anti-SGK3 antibody with human islet of langerhans at 1 ug/ml.

Immunohistochemistry-Paraffin: SGK3 Antibody [NLS7240] - Anti-SGK3 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Discontinued

SGK3 Antibody Summary

• Immunogen: Synthetic 20 amino acid peptide from internal region of human SGK3.
• Localization: Cytoplasmic vesicle (By similarity). Early endosome (By similarity).
• Specificity: Human SGK3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Chicken (90%), Monkey (95%), Primate (95%), Turkey (90%), Equine (95%), Bovine (95%), Orangutan (100%), Rabbit (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 36 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (95%), Opossum (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SGK3 Antibody

• CISK
• DKFZp781N0293
• EC 2.7.11
• EC 2.7.11.1
• serine/threonine-protein kinase Sgk3
• serum/glucocorticoid regulated kinase 3
• serum/glucocorticoid regulated kinase family, member 3
• serum/glucocorticoid regulated kinase-like
• Serum/glucocorticoid-regulated kinase 3
• Serum/glucocorticoid-regulated kinase-like
• SGK2
• SGK3
• SGKLcytokine-independent survival kinase

Background

SGKL/SGK3, also known as CISK for cytokine-independent survival kinase, is a SGK type protein kinase. The protein contains a lipid-binding phox homology (PX) domain, which is required for targeting SGKL/SGK3 protein to the endosomal compartment. SGKL/SGK3 functions downstream of the phosphoinositide 3-kinase (PI3K) cascade and has been implicated in cell survival. The protein has been shown to be phosphorylated and activated by 3-phosphoinositide-dependent protein kinase 1 (PDK1), and to a lesser extent insulin-like growth factor-1, in vitro. Activated SGKL/SGK3 has been shown to phosphorylate glycogen synthase kinase 3 beta (GSK-3beta) in vitro. SGKL/SGK3 expression has been documented in many human tissues but was found to be most abundant in lung. ESTs have been isolated from human tissue libraries, including normal liver, breast and thyroid and cancerous spleen, ear and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SGK3 Antibody (NLS7240)(1)

Publication using NLS7240

• Yao LJ, McCormick JA, Wang J et al. Novel role for SGK3 in glucose homeostasis revealed in SGK3/Akt2 double-null mice. Mol Endocrinol. 2011-12-01 [PMID: 21980074] (IHC-P, Mouse)
  
  Details:
  SGkL/SGk3 (IMG-71950). IHC (paraffin): Pancreas from Akt2+/+/SGk3+/- transgenic mice, Fig 8A.

Bioinformatics

• Gene Symbol: SGK3
• Entrez:
  • 27219(Mouse)
  • 171498(Rat)
• Uniprot:
  • Q96BR1(Human)