SIAH1 Antibody

Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SIAH1 Antibody Summary

• Immunogen: Peptide with sequence SRQTATALPTGTSKC corresponding to N-Terminus according to NP_003022.3, NP_001006611.1.
• Specificity: This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1).
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIAH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:128000
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID: 19479934).
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIAH1 Antibody

• E3 ubiquitin-protein ligase SIAH1
• EC 6.3.2
• EC 6.3.2.-
• FLJ08065
• hSIAH1
• HUMSIAH
• seven in absentia homolog 1 (Drosophila)
• Seven in absentia homolog 1
• Siah-1
• SIAH1A
• siah-1a

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIAH1 Antibody (NB300-974)(6)

Publications using NB300-974

• Sroka K, Voigt A, Deeg S, Reed JC, Schulz JB, Bahr M, Kermer P. BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution. J Neurochem;111(3):801-7. 2009-11-01 [PMID: 19712056]
• Liman et al. Molecules, 15, 6678-6687. 2010 [PMID: 19479934] (IP)
• Guigon CJ, Zhao L, Lu C, Willingham MC, Cheng SY. Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor. Mol Cell Biol;28(14):4598-608. 2008-07-01 [PMID: 18474620]
• Groenman F, Rutter M, Caniggia I et al. Hypoxia-inducible factors in the first trimester human lung. J Histochem Cytochem 2007-04-01 [PMID: 17189520]
• Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno K, Chiba T. Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner. Oncogene;23(45):7593-600. 2004-09-30 [PMID: 15326481]
• Susini L, Passer BJ, Amzallag-Elbaz N et al. Siah-1 binds and regulates the function of Numb. Proc Natl Acad Sci U S A 2001-12-18 [PMID: 11752454]

Bioinformatics

• Gene Symbol: SIAH1
• Entrez:
  • 6477(Human)
  • 20437(Mouse)
  • 140941(Rat)
• Uniprot:
  • Q8IUQ4(Human)