Siglec-2/CD22 Antibody (RFB4) [PE]

Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [PE] [NBP2-49890PE] - Flow cytometric analysis of a normal blood sample after immunostaining with Siglec-2/CD22 Antibody (RFB4) [PE].

Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [PE] [NBP2-49890PE] - Flow cytometric analysis of a normal blood sample after immunostaining with Siglec-2/CD22 Antibody (RFB4) [PE].

Product Discontinued

Siglec-2/CD22 Antibody (RFB4) [PE] Summary

• Description: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
• Immunogen: 135 kDa type I integral membrane protein
• Specificity: This antibody recognizes surface Siglec-2/CD22 expressed by mature peripheral B-cells and cytoplasmatic Siglec-2/CD22 expressed by precursor B-cells. The sensitivity is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD22
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
• Application Notes: .

Reactivity Notes

Use in Canine reported in scientific literature (PMID:31826004).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) and 1.0% (w/v) BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Siglec-2/CD22 Antibody (RFB4) [PE]

• B-cell receptor CD22
• BL-CAM
• B-lymphocyte cell adhesion molecule
• CD22 antigenMGC130020
• CD22 molecule
• CD22
• sialic acid binding Ig-like lectin 2
• Sialic acid-binding Ig-like lectin 2
• Siglec2
• Siglec-2
• SIGLEC2FLJ22814
• T-cell surface antigen Leu-14

Background

The epitope recognized by antibody RFB4 is a 135 kDa type I integral membrane protein expressed by human B-cells. Precursor B-cells are surface-CD22 negative, but cytoplasmic CD22 positive. Mature B-lymphocytes express CD22 also on their surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-2/CD22 Antibody (NBP2-49890PE)(1)

Publication using NBP2-49890PE

• Craig KKL, Wood GA, Keller SM, et al. MicroRNA profiling in canine multicentric lymphoma PLoS One. [PMID: 31826004] (FLOW, Canine)

FAQs for Siglec-2/CD22 Antibody (NBP2-49890PE). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
   • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Bioinformatics

• Gene Symbol: CD22