Siglec-7/CD328 Antibody (194211) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human Siglec-7/CD328 Monoclonal Antibody (Catalog # MAB11381) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 194211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-7/CD328 Antibody (194211) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-7
  Gln19-Gly357 (predicted)
  Accession # Q9Y286
• Specificity: Detects human Siglec-7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-6, -8, -9 or recombinant Siglec-E is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-7/CD328 Antibody (194211) [Unconjugated]

• Adhesion inhibitory receptor molecule 1
• adhesion inhibitory receptor molecule 1, siglec-7
• AIRM1
• AIRM-1
• AIRM1QA79 membrane protein
• CD328 antigen
• CD328
• CDw328
• D-siglec
• p75
• p75/AIRM1
• QA79
• sialic acid binding Ig-like lectin 7
• sialic acid binding immunoglobulin-like lectin 7
• sialic acid-binding Ig-like lectin 7
• Siglec7
• Siglec-7

Background

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. The cDNA of human Siglec-7, also known as adhesion inhibitory receptor module-1 (AIRM-1) and designated CD328, encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
5. Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-7/CD328 Antibody (MAB11381)(1)

Publication using MAB11381

• Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike W. Looman, Erik Hooijberg, Christian Büll, Daniel Granado et al. Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7 Cellular and Molecular Life Sciences 2023-06-01 [PMID: 37253806]

Bioinformatics

• Gene Symbol: SIGLEC7
• Uniprot:
  • Q9Y286()