Recombinant Single-stranded DNA Binding Protein

SDS-Page: Recombinant Human, Other Single-stranded DNA Binding Protein [NBP2-35314] - Single-stranded DNA Binding Protein [NBP2-35314]

• Applications: PAGE
• Format: Carrier-Free

Recombinant Single-stranded DNA Binding Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 148 amino acids corresponding to Single-stranded DNA Binding ProteinSource: E. coli

  Uniprot ID: Q97W73

  Amino Acid Sequence: MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAPQQMRG GGRGFRGGGR RYGRRGGRRQ ENEEGEEE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Single-stranded DNA Binding as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 16.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered concentrated solution in 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM EDTA, 0.5 mM DTT.
• Preservative: 50% glycerol
• Purity: >95%, by SDS-PAGE and HPLC

Background

Single-stranded DNA-binding protein, or SSB, binds to single-stranded regions of DNA to prevent premature annealing, to protect the single-stranded DNA from being digested by nucleases, and to remove secondary structure from the DNA to allow other enzymes to function effectively upon it. In molecular biology, SSB protein domain in bacteria are important in its function of maintaining DNA metabolism, more specifically DNA replication, repair and recombination. SSB proteins have been identified in organisms from viruses to humans. The only organisms known to lack them are thermoproteales, a group of extremophile archaea, where they have been displaced by the protein ThermoDBP. While many phage and viral SSBs function as monomers and eukaryotes encode heterotrimeric RPA (Replication Protein A), the best characterized SSB is that from the bacteria E. coli which, like most bacterial SSBs exists as a tetramer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Uniprot:
  • Q97W73(Other)