Smith Antigen Antibody (Y12)

Western Blot: Smith Antigen Antibody (Y12) [NB600-546] - Analysis of LS174T Cells.

Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546] - Human tonsil stained with Smith antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

• Reactivity: Hu, Mu, Rt, Po, Dr, Rb
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: Y12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Smith Antigen Antibody (Y12) Summary

• Immunogen: The immunogen is generated from human Smith Antigen.
• Localization: Nuclear
• Specificity: Ab-1 recognizes cross-reactive epitopes on the B'/B and D polypeptides of Sm. Ab-1 precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Immunomicroscopy 1:10 - 1:500
  • Western Blot 0.5 - 1.0 ug/ml
• Application Notes: WB: Detects an approx. 25 kDa, which corresponds to the predicted molecular weight of Smith Antigen.Precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all of these RNA-protein complexes. Use in ELISA reported in scientific literature (PMID 28093526). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28515487).

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 17473171). Canine (PMID: 10330151). Mouse reactivity reported in scientific literature (PMID: 28093526).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for Smith Antigen Antibody (Y12)

• Smith antigen

Background

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smith Antigen Antibody (NB600-546)(7)

Publications using NB600-546

• Andrey Damianov, Chia-Ho Lin, Jeffrey Huang, Lin Zhou, Yasaman Jami-Alahmadi, Parham Peyda, James Wohlschlegel, Douglas L Black The splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. Molecular cell 2024-04-22 [PMID: 38537639]
• Esser L, Li Q, Jüdt M et al. The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis International Journal of Molecular Sciences 2023-10-25 [PMID: 37958537] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• M Schilling, AB Prusty, B Boysen, FS Oppermann, YL Riedel, A Husedzinov, H Rasouli, A König, P Ramanathan, J Reymann, H Erfle, H Daub, U Fischer, OJ Gruss TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis Cell Reports, 2021-06-22;35(12):109277. 2021-06-22 [PMID: 34161763]
• Naro C, Pellegrini L et al. Functional Interaction between U1snRNP and Sam68 Insures Proper 3' End Pre-mRNA Processing during Germ Cell Differentiation. Cell Rep 2019-12-03 [PMID: 30865884] (WB, Mouse)
• Mirra A, Rossi S, Scaricamazza S et al. Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. Sci Rep 2017-05-17 [PMID: 28515487] (ICC/IF, IF/IHC, Mouse)
• Zhou Y, Chen H, Liu L et al. Cathepsin K Deficiency Ameliorates Systemic Lupus Erythematosus-like Manifestations in Faslpr Mice J. Immunol 2017-01-16 [PMID: 28093526] (ELISA, Mouse)
• Somarelli JA, Mesa A, Rodriguez R, Avellan R, Martinez L, Zang YJ, Greidinger EL, Herrera RJ. Epitope mapping of the U1 small nuclear ribonucleoprotein particle in patients with systemic lupus erythematosus and mixed connective tissue disease. Lupus;20(3):274-89. 2011-03-01 [PMID: 21362751] (WB, Human)