Smoothelin Antibody (885424) [Unconjugated] - B

Smoothelin B was detected in formalin fixed paraffin-embedded sections of human colon using Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HASMC human aortic smooth muscle cells was stained with Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: IHC, CyTOF-ready, Flow
• Clone: 885424
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Smoothelin Antibody (885424) [Unconjugated] - B Summary

• Immunogen: E. coli-derived recombinant human Smoothelin B
  Met1-Leu132
  Accession # P53814
• Specificity: Detects human Smoothelin B in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMTN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smoothelin Antibody (885424) [Unconjugated] - B

• FLJ35365
• FLJ38597
• Smoothelin
• SMSMO
• SMTN

Background

Smoothelin (SMTN) is a cytoskeletal protein associated with stress fibers.  It is 917 amino acids (aa) in length and shares 77% and 82% aa identity with mouse and rat SMTN.  SMTN has two major isoforms, A and B with reported molecular weights of approximately 59 and 110 kDa, respectively.  Isoform A is associated with visceral smooth muscle cells while isoform B is specific to vascular smooth muscle.  Loss of SMTN has been associated with defects in intestinal smooth muscle cell contraction as well as increased arterial pressure and cardiac hypertrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMTN
• Uniprot:
  • P53814()