SOCS-1 Antibody

Immunocytochemistry/Immunofluorescence: SOCS-1 Antibody [NB100-781] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Negative Control showing staining of paraffin embedded Mouse Kidney, with no primary antibody.

Flow Cytometry: SOCS-1 Antibody [NB100-781] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Staining of paraffin embedded Mouse Kidney. Antibody at 10 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining (1:500).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SOCS-1 Antibody Summary

• Immunogen: Peptide with sequence C-VLRDYLSSFPFQI corresponding to C-Terminus according to NP_003736.1.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOCS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
• Control:

  Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
  SOCS-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOCS-1 Antibody

• CIS1
• Cish1
• JAB
• JABTec-interacting protein 3
• JAK binding protein
• JAK-binding protein
• SOCS1
• SOCS-1
• SOCS-1CIS1
• SSI-1
• SSI-1CISH1
• SSI1cytokine-inducible SH2 protein 1
• STAT induced SH3 protein 1
• STAT-induced STAT inhibitor 1
• suppressor of cytokine signaling 1
• TIP3
• TIP-3
• TIP3Cish1

Background

Cytokines induce a variety of biologic responses by binding to specific cell surface receptors and activating cytoplasmic signal transduction pathways. Proteins that mediate these signals from the cell surface to the nucleus include Janus kinases, e.g., Janus kinase-1, and the signal transducers and activators of transcription (STATs), such as STAT1. To maintain tight control of these signal transduction pathways, mechanisms for turning off the signals are necessary. The adverse consequences of an imbalance between positive and negative signals is evident in the 'motheaten' mouse, which lacks the phosphatase SHP1. These mice fail to regulate cytokine responses appropriately, resulting in hyperproliferation and accumulation of cells in several hematopoietic lineages and the development of systemic autoimmune disease. SOCS1 represents a family of proteins that regulates responses to cytokines. SOCS1 (also known as Janus kinase-binding protein and STAT-induced STAT inhibitor-1) was isolated independently by its ability to interact with Janus kinase-2 in a 2-hybrid screen and on the basis of antigenic similarity with the SH2 domain of STAT3. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine-inducible SH2-containing protein).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-1 Antibody (NB100-781)(3)

Publications using NB100-781

• Jenny L. Wilkerson, Lauren B. Alberti, Ganesh A. Thakur, Alexandros Makriyannis, Erin D. Milligan Peripherally administered cannabinoid receptor 2 (CB 2 R) agonists lose anti-allodynic effects in TRPV1 knockout mice, while intrathecal administration leads to anti-allodynia and reduced GFAP, CCL2 and TRPV1 expression in the dorsal spinal cord and DRG Brain research 2024-01-03 [PMID: 34774500]
• Wilkerson JL, Alberti LB, Kerwin AA et al. Peripheral versus central mechanisms of the cannabinoid type 2 receptor agonist AM1710 in a mouse model of neuropathic pain Brain Behav 2020-09-25 [PMID: 32977358]
• Starr R, Willson TA, Viney EM et al. A family of cytokine-inducible inhibitors of signalling. Nature 1997-06-26 [PMID: 9202125]

Bioinformatics

• Gene Symbol: SOCS1
• Entrez:
  • 8651(Human)
  • 12703(Mouse)
• Uniprot:
  • O15524(Human)