Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

SDS-Page: Recombinant Human Sodium Potassium ATPase Beta 1 Protein [NBP2-59894] - 15% SDS-PAGE

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Human Sodium Potassium ATPase Beta 1 His Protein Summary

• Description: A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 63-303 of Human Sodium Potassium ATPase Beta 1
  
  

  Source:Baculovirus

  Amino Acid Sequence: ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH

• Details of Functionality: Specific activity is > 3,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25C.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: ATP1B1
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

• adenosinetriphosphatase
• ATP1B
• ATP1B1
• ATPase, Na+/K+ transporting, beta 1 polypeptide
• Beta 1-subunit of Na(+)
• K(+)-ATPase
• MGC1798
• Na, K-ATPase beta-1 polypeptide
• Sodium Potassium ATPase Beta 1
• sodium/potassium-dependent ATPase beta-1 subunit
• Sodium/potassium-dependent ATPase subunit beta-1
• sodium/potassium-transporting ATPase beta-1 chain
• sodium/potassium-transporting ATPase subunit beta-1

Background

Sodium Potassium ATPase Beta 1 (ATP1B1) is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. Na/K ATPase pumps consist of alpha/beta heterodimers. Beta 1 is the glycosylated, non-catalytic component of the active enzyme responsible for the formation of these alpha/beta heterodimers. Through this assembly, the beta polypeptide regulates the number of sodium pumps transported to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP1B1