Src Antibody (1B9)

Immunocytochemistry/Immunofluorescence: Src Antibody (1B9) [H00006714-M01] - Analysis of monoclonal antibody to SRC on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Src Antibody (1B9) [H00006714-M01] - Analysis of monoclonal antibody to SRC on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.

Proximity Ligation Assay: Src Antibody (1B9) [H00006714-M01] - Analysis of protein-protein interactions between PTK2 and SRC. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-SRC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Src Antibody (1B9) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SRC (AAH11566, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFV
• Specificity: SRC - v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (1B9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRC
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Src Antibody (1B9)

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRC
• Entrez:
  • 6714(Human)
• Uniprot:
  • AAH11566(Human)