Src Antibody (1F11)

Western Blot: Src Antibody (1F11) [NBP2-37543] - Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.

Immunohistochemistry: Src Antibody (1F11) [NBP2-37543] - Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC mouse mAb with DAB staining.

Flow Cytometry: Src Antibody (1F11) [NBP2-37543] - Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).

Western Blot: Src Antibody (1F11) [NBP2-37543] - Western blot analysis using SRC mAb against human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)

Immunohistochemistry: Src Antibody (1F11) [NBP2-37543] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC mouse mAb with DAB staining.

ELISA: Src Antibody (1F11) [NBP2-37543] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

Src Antibody (1F11) Summary

• Immunogen: Purified recombinant fragment of human SRC expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Src Antibody (1F11)

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRC