Src Antibody (5A18) - BSA Free

Western Blot: Src Antibody (5A18) [NBP1-19188] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot. From a verified customer review.

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.

Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human adrenal tissue stained with Src, mAb (5A18) at 20 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 5A18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Src Antibody (5A18) - BSA Free Summary

• Immunogen: Recombinant human full length c-Src
• Specificity: Src (5A18)
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western
  • Western Blot 1 ug/ml
• Application Notes: WB validation from a verified customer review.
  See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Src Antibody (5A18) - BSA Free

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a member of the tyrosine kinase family that was originally identified as the oncogene of Rous sarcoma virus. The catalytic domain contains a tyrosine autophosphorylation site that increases kinase activity when phosphorylated. The Src family of tyrosine kinase is involved in a variety of receptor-induced signal transduction pathways such as immune recognition rec

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Src Antibody (NBP1-19188)(2)

Publications using NBP1-19188

• Bubmann L, Hoffer K, von Bargen CM et al. Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets International journal of cancer 2021-04-22 [PMID: 33890294]
• Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca 2+ Release Stimulates Breast Cancer Stem Cell Enrichment Cell Rep. 2017-02-21 [PMID: 28228260] (WB, Human)
  
  Details:
  The positive impact of HIF inhibitors on breast cancer chemotherapy is explored through GSTO1 knockdown.

Reviews for Src Antibody (NBP1-19188) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 10/11/2018

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: 12180625

reviewed by: Hongxia Hu WB Human 09/12/2012

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: SRC
• Entrez:
  • 6714(Human)
  • 20779(Mouse)
  • 83805(Rat)
• Uniprot:
  • P12931()