SREBP1 Antibody

Western Blot: SREBP1 Antibody [NB100-60545] - Detection of SREBP1 in human liver (40 ug) using NB100-60545.

Product Discontinued

SREBP1 Antibody Summary

• Immunogen: A synthetic peptide made to a portion of the human SREBP1 protein sequence (between residues 700-800). [Uniprot: P36956]
• Localization: Integral membrane protein that moves from ER to Golgi in absence of sterols.
• Predicted Species: Chinese Hamster (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SREBF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
• Application Notes: This SREBP1 antibody is useful for Western blot, where a band is seen at ~65 kDa (mature form).
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SREBP1 Peptide (NB100-60545PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: porcine (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Immunogen affinity purified

Notes

Unprocessed SREBP1 is an ~122 kDa integral membrane protein that moves from ER to golgi for processing. The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi. This is the ~65 kDa mature form that is detected by NB100-2215. The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65 kDa protein by NB100-60545.

Alternate Names for SREBP1 Antibody

• bHLHd1
• Class D basic helix-loop-helix protein 1
• SREBF1
• SREBP 1c
• SREBP1
• SREBP-1
• SREBP1bHLHd1SREBP-1c
• sterol regulatory element binding protein-1
• sterol regulatory element binding transcription factor 1
• sterol regulatory element-binding protein 1
• Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SREBP1 Antibody (NB100-60545)(6)

Publications using NB100-60545

• Ding Y, Xu X, Meng B et al. Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKK?/NF-?B signaling Cell death & disease 2023-06-26 [PMID: 37365185] (WB, Mouse)
  
  Details:
  1:1000 dilution
• McMurphy TB. Environmental and gene therapy approaches to improve glycemic control and promote healthy aging. Thesis. 2017-10-26 (WB, Mouse)
• Rana, R, Shearer, A M Et al. PAR2 controls cholesterol homeostasis and lipid metabolism in nonalcoholic fatty liver disease. Mol Metab 2019-11-01 [PMID: 31668396] (ICC/IF, Drosophila melanogaster)
• Pathak, P;Chiang, JYL; Sterol 12 alpha-hydroxylase Aggravates Dyslipidemia by Activating the Ceramide/mTORC1/SREBP1C Pathway via FGF21 and FGF15 Gene Expr. 2019-03-19 [PMID: 30890204] (WB, Mouse)
• McMurphy T, Huang W, Queen NJ et al. Implementation of environmental enrichment after middle age promotes healthy aging Aging (Albany NY) 2018-07-20 [PMID: 30036185] (WB, Mouse)
• McMurphy TB, Huang W, Xiao R et al. Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism Through AKT Activation Diabetes. 2016-11-29 [PMID: 27903748] (WB, Mouse)

FAQs for SREBP1 Antibody (NB100-60545). (Showing 1 - 2 of 2 FAQs).

1. I am looking for SREBF1 antibodies that can cross-react with zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebrafish or have high sequence identity in the immunogen region.
   • Unfortunately we do not have any antibodies that have been tested in zebrafish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebrafish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovators Reward Program. NB600-582 - 301-407 aa, NB100-2215 - 300-400 aa, NB100-60545 - 700-800 aa.
2. A customer is interested in NB100-60545 for use with zebra fish. Would you be able to blast the exact immunogen sequence to see what the homology is?
   • The homology for the immunogen of this antibody is approximately 78%.

Bioinformatics

• Gene Symbol: SREBF1