SSEA-4 Antibody (MC-813-70) [NL557]

The stem cell marker SSEA-4 was visualized in live BG01V human embryonic stem cells using a GloLIVE NL557-conjugated Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # NLLC1435R; red). The cells were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-4 in human stem cells is an indicator of pluripotency.

• Reactivity: Hu, Mu
• Applications: ICC/IF
• Clone: MC-813-70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: NL557

SSEA-4 Antibody (MC-813-70) [NL557] Summary

• Additional Information: 50X Concentration, Azide Free
• Specificity: Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-4 Antibody (MC-813-70) [NL557]

• SSEA4
• SSEA-4
• SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

1. Shevinsky, L.H. et al. (1982) Cell 30:697.
2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.