Human STAT3 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Human STAT3 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The STAT3/LUCPorter™ Reporter Cell Line is suitable for monitoring the transcriptional activity of STAT3. This cell line can be used for studying the STAT3-mediated signaling pathways as well as screening of agonists/antagonists/signaling inhibitors related with STAT3 phosphorylation-dependent cell activation.
  
  As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines including IL-6, interferons, epidermal growth factor, hepatocyte growth factor and leukemia inhibitory factor. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. STAT3 plays an important role in cellular processes such as cell cycle progression and apoptosis. Constitutive activation of STAT3 is associated with malignant transformation and tumor progression of various cancers, so that STAT3 has been issued as a novel cancer drug target.
  
  Functional activity of the STAT3/LUCPorter ™ HEK 293 Reporter Cell Line has been validated by induction of STAT3-responsive luciferase activity by IL-6 together with different cell reagents such as piceatannol, which selectively inhibits the tyrosine phosphorylation of STAT3, curcumin (NBP2-26243), which is a known inhibitor for IL-6-mediated STAT3 phosphorylation, and sodium pervanadate, the tyrosine phosphatase inhibitor (Figures 1, 2 and 3).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The STAT3/LUCPorter™ HEK 293 Reporter Cell Line is a stably transfected cell line which expresses an optimized Renilla luciferase reporter gene under the transcriptional control of the STAT3 response element.
• Target Species: Human
• Specificity: STAT3 LUCPorter™
• Selection Agent: Puromycin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Reporter Gene: Renilla luciferase
• Host: HEK293
• Gene: STAT3

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Antagonist:
  • Curcumin NBP2-26243
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-25287
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the STAT3 reporter cell line.
- Wash hands after handling the STAT3 reporter cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with the cell line.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human STAT3 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• Acute-phase response factor
• APRFMGC16063
• DNA-binding protein APRF
• FLJ20882
• HIES
• signal transducer and activator of transcription 3 (acute-phase responsefactor)
• signal transducer and activator of transcription 3
• STAT3

Background

Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3 is activated by phosphorylation on tyrosine 705 which induces dimerization, nuclear translocation, and DNA binding While, Stat3-activated transcription seems to be regulated by serine phosphorylation at serine 727 (4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Publications for STAT3 Reporter Cell Line (NBP2-26254)(1)

Publication using NBP2-26254

• Lamichhane P, Karyampudi L, Shreeder B, Krempski J et al. IL10 Release upon PD-1 Blockade Sustains Immunosuppression in Ovarian Cancer Cancer Res. 2017-10-09 [PMID: 28993412] (WB, Mouse)

Bioinformatics

• Gene Symbol: STAT3