Staufen Antibody (3C12)

Immunocytochemistry/Immunofluorescence: Staufen Antibody (3C12) [H00006780-M03] - Analysis of monoclonal antibody to STAU1 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: Staufen Antibody (3C12) [H00006780-M03] - Detection limit for recombinant GST tagged STAU1 is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Staufen Antibody (3C12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: STAU1 (NP_004593, 401 a.a. ~ 496 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC
• Localization: Rough endoplasmic reticulum
• Specificity: STAU1 - staufen, RNA binding protein, homolog 1 (Drosophila)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAU1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Staufen Antibody (3C12)

• double-stranded RNA-binding protein Staufen homolog 1
• FLJ25010
• RNA binding protein (Drosophila)
• RNA-binding protein)
• staufen, RNA binding protein, homolog 1 (Drosophila)

Background

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAU1
• Entrez:
  • 6780(Human)
• OMIM:
  • 601716(Human)
• Uniprot:
  • NP_004593(Human)