Streptavidin Antibody (S10D4)

Product Discontinued

Streptavidin Antibody (S10D4) Summary

• Immunogen: Streptavidin.
• Specificity: No cross-reactivity with egg white avidin. Binds with high efficiency with different epitopes of streptavidin molecule. Binds to the streptavidin without interfering with the biotin-binding reaction.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Can be used to localize streptavidin, to amplify signals in immunochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes. WB: Use at an assay dependent dilution. Predicted molecular weight: 19 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

All Species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Streptavidin Antibody (S10D4)

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.