Streptavidin Antibody (S8C12cc) - BSA Free

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
S8C12cc
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

Streptavidin Antibody (S8C12cc) - BSA Free Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant streptavidin.
Specificity
No cross-reactivity with egg white avidin. Binds to streptavidin without interfering with the biotin-binding reaction.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
Application Notes
Can be used to localize streptavidin, to amplify signals in immunocytochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Streptavidin Antibody (S8C12cc) - BSA Free

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAb S8C12 reacts with the determinant nonoverlapping with S8E4-, S10D4- and S3E11-epitopes. All MAbs bind to the streptavidin without interfering with the biotin-binding reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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