Human Survivin ELISA Kit (Colorimetric)

ELISA: Survivin ELISA Kit [Biotin] [KA0441] - Standard curve

Product Discontinued

Human Survivin ELISA Kit (Colorimetric) Summary

• Description: BIRC5 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human BIRC5.
• Standard Curve Range: 62.5 to 4000 pg/mL
• Sensitivity: <2 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BIRC5

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Survivin ELISA Kit (Colorimetric)

• API4
• apoptosis inhibitor 4
• Apoptosis inhibitor survivin
• baculoviral IAP repeat-containing 5
• Baculoviral IAP Repeat-Containing Protein 5
• BIRC5
• EPR-1
• IAP4
• survivin variant 3 alpha
• Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Survivin ELISA Kit (KA0441)(5)

Publications using KA0441

• Chen D, Xu J, Zhang Q. et al. Detection of survivin expression in bladder cancer and renal cell carcinoma using specific monoclonal antibodies. Oncol Rep 2018-04-05 [PMID: 29658613]
• Jia X, Gao Y, Zhai D et al. Survivin is not a promising serological maker for the diagnosis of hepatocellular carcinoma. Oncol Lett 2015-05-01 [PMID: 26137069]
• Blanc-Brude OP, Yu J, Simosa H et al. Inhibitor of apoptosis protein survivin regulates vascular injury. Nat Med. 2002-09-01 [PMID: 12172543]
• Li F, Ambrosini G, Chu EY et al. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 1998-12-10 [PMID: 9859993]
• Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 1997-08-01 [PMID: 9256286]

FAQs for Survivin ELISA Kit (KA0441). (Showing 1 - 4 of 4 FAQs).

1. Can I use this antibody with species other than those listed?
   • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
2. What is the standard curve range for this kit?
   • The standard curve range is 62.5 to 4000 pg/mL for this Survivin kit.
3. What sample types can I use this with?
   • The suitable sample types we have listed consist of Body Fluid, Cell Culture Supernatant, Plasma, Serum, and Tissue Lysate.
4. Can this be used with non-human species?
   • This kit has not been validated for any species besides human, so we cannot guarantee that this will work with non-human species.

Bioinformatics

• Gene Symbol: BIRC5