T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack

Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).

Flow Cytometry: T Cell Exhaustion Marker (Flow-Directly Conjugated) - Human peripheral blood mononuclear cells (PBMCs) gated on CD3(-) cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog# FAB2408P) and either (A) Mouse Anti-Human TIGIT APC-conjugated Monoclonal Antibody (Catalog # FAB7898A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog# IC0041A).

• Reactivity: Hu
• Applications: Flow
• Clonality: Monoclonal

T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack Summary

• Description: The Human T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Sampler Pack contains antibodies to detect expression of inhibitory receptors associated with T cell exhaustion. T cells co-expressing these inhibitory receptors are found during chronic viral infection and within the TME of many solid tumors. Anti-PD-1 is directly conjugated to PE, while anti- CTLA-4, TIGIT, TIM-3, VISTA/B7-H5/PD-1H and LAG-3 are directly conjugated to APC. This pack contains 6 full sized vials of directly conjugated primary antibody. It will allow the user to determine the co-expression of emerging checkpoint targets like LAG-3, TIM-3, TIGIT, and VISTA with the established checkpoint target, PD-1 using flow cytometry. All antibodies have been validted for use in flow cytometry applications.
  
  This pack contains 1 vial each of:
  FAB10863P (100 ug)
  FAB3253A (100 ug)
  FAB7898A (100 ug)
  FAB2365RA (0.1 ml)
  MAB71263APC (100 ul)
  MAB23191APC (100 ul)
• Immunogen: See individual datasheets
• Marker: T cell exhaustion
• Specificity: Please see each vial's data sheet for specificity information specific to each product.
• Clonality: Monoclonal

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Storage is content dependent.
• Buffer: See individual datasheets

Alternate Names for T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack

• Immune cell exhaustion markers
• immune checkpoint markers
• inhibitory receptors
• T cell exhaustion markers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.