Talin1 Antibody (97H6) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 97H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Talin1 Antibody (97H6) - BSA Free Summary

• Immunogen: His-tagged polypeptide containing residues 489-911 of mouse Talin1
• Specificity: Does not cross react with related Talin2 protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): Fibroblasts.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Talin1 Antibody (97H6) - BSA Free

• ILWEQ
• KIAA1027
• talin 1
• Talin1
• talin-1
• TLN
• TLN1

Background

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Talin1 Antibody (NBP2-50320)(4)

Publications using NBP2-50320

• Miihkinen M, GrOnloh M, Vihinen H et al. Myosin-X is required for integrin activation at filopodia tips bioRxiv 2020-05-06 (WB, Human)
• Isomursu A, Park KY, Hou J et al. Directed cell migration towards softer environments Nature materials 2022-07-11 [PMID: 35817964] (WB, Human)
• Miihkinen M, GrOnloh MLB, Popovic A et al. Myosin-X and talin modulate integrin activity at filopodia tips Cell reports 2021-09-14 [PMID: 34525374]
• Pedrosa AT, Murphy KN, Nogueira AT et al. A post-invasion role for Chlamydia type III effector TarP in modulating the dynamics and organization of host cell focal adhesions J. Biol. Chem. 2020-08-25 [PMID: 32843479] (ICC/IF, Human, Mouse, Monkey)

Bioinformatics

• Gene Symbol: TLN1