Talin1 Antibody (TA205) - BSA Free

Flow Cytometry: Talin1 Antibody (TA205) [NBP1-21642] - Human peripheral blood platelets stained with mouse anti-human Talin1.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP
• Clone: TA205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Talin1 Antibody (TA205) - BSA Free Summary

• Immunogen: Human platelet talin
• Specificity: Immunoblots the 225 kDa talin band on SDS gels of platelets and other cells. It may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. TA205 recognizes an epitope within residues 139-433.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Flow Cytometry - Membrane permeabilization is required.

Reactivity Notes

Predicted cross-reactivities: Chicken, Rabbit

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Talin1 Antibody (TA205) - BSA Free

• ILWEQ
• KIAA1027
• talin 1
• Talin1
• talin-1
• TLN
• TLN1

Background

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Talin1 Antibody (NBP1-21642)(1)

Publication using NBP1-21642

• Longo G, Ioannidu CA, Scotto d'Abusco A et al. Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon. PLoS ONE. 2016-04-01 [PMID: 27031101] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: TLN1
• Entrez:
  • 7094(Human)
• Uniprot:
  • Q9Y490(Human)