TAP Tag Antibody (22F12-1E3)

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891] - WB analysis of TAP tag.

Western Blot: TAP Tag Antibody (22F12-1E3) [NBP2-54891] - WB analysis of TAP tag.

• Reactivity: Hu, All-NA
• Applications: WB, IHC
• Clone: 22F12-1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TAP Tag Antibody (22F12-1E3) Summary

• Immunogen: KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000 - 1:2000
• Application Notes: Detects TAP in WB applications. The immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.

Reactivity Notes

TAG

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA and 30% glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TAP Tag Antibody (22F12-1E3)

• tandem affinity purification tag
• tandem affinity purification
• TAP

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.