TATA binding protein TBP Antibody

Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TATA binding protein TBP Antibody Summary

• Immunogen: Peptide with sequence C-TPQPIQNTNSLS corresponding to internal region according to NP_001165556.1.
• Epitope: CTPQPIQNTNSLS
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003185.1; NP_001165556.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in nuclear, but not in cytosolic HeLa lysates (calculated MW of 37.7 kDa band according to NP_003185.1) and 37 kDa band observed in lysates of NIH3T3 and of mouse and rat testis lysates (calculated MW of 34.7 kDa band according to mouse NP_038712.3 and of 35.0 kDa band according to rat NP_001004198.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TATA binding protein TBP Antibody

• GTF2D
• GTF2D1
• HDL4
• MGC126054
• MGC126055
• SCA17
• TATA box binding protein
• TATA sequence-binding protein
• TATA-binding factor
• TATA-box binding protein N-terminal domain
• TATA-box factor
• TATA-box-binding protein
• TF2D
• TFIIDMGC117320
• Transcription initiation factor TFIID TBP subunit

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein TBP and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.TPB is also known to interact with HIV1 Tat protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TATA binding protein TBP Antibody (NBP2-12933)(1)

Publication using NBP2-12933

• Pessina F, Giavazzi F, Yin Y et al. Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors. Nature cell biology 2020-02-11 [PMID: 31570834]

Bioinformatics

• Gene Symbol: TBP
• Entrez:
  • 6908(Human)
  • 21374(Mouse)
  • 117526(Rat)