Tau Antibody (TAU-5)

Product Discontinued

Tau Antibody (TAU-5) Summary

• Immunogen: Purified bovine microtubule-associated proteins.
• Epitope: Epitope maps in the middle of tau.
• Localization: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
• Marker: pan-Neuronal Marker
• Specificity: This antibody recognizes proteins of 45-68 kDa, identified as tau proteins. Tau proteins promote the assembly of tubulin monomers into microtubules and stabilize microtubules. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau. The TAU-5 monoclonal antibody reacts with the non-phosphorylated as well as the phosphorylated forms of tau. Its epitope is located in the middle region of tau. This antibody is highly specific for tau and does not cross-react with other microtubule associated proteins (MAPs) or tubulin. In immunohistology, this antibody intensely stains the human neurofibrillary tangles, neuropil threads, and neuritic plaques associated with Alzheimer's disease. This antibody is also observed to stain astrocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1/25 - 1/50
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1/50 - 1/100
• Application Notes: This antibody is suitable for use in Western blotting. The optimal antibody concentration should be determined for each specific application.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Alzheimer's)
  Tau Overexpression Lysate

Reactivity Notes

Cross-reacts with human, sheep, cow, mouse, and rat tau. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Tau Antibody (TAU-5)

• DDPAC
• FLJ31424
• FTDP-17
• G protein beta1/gamma2 subunit-interacting factor 1
• MAPT
• MGC138549
• microtubule-associated protein tau
• MSTD
• MSTDMAPTL
• MTBT1
• MTBT1Neurofibrillary tangle protein
• MTBT2
• Neurofibrillary tangle protein
• PHF-tau
• PPND
• Tau
• TAUPaired helical filament-tau

Background

Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tau Antibody (NB200-514)(1)

Publication using NB200-514

• Ni JN, Shi J, Zhang XK et al. Effect of GAPT extract on expression of tau protein and its phosphorylation related enzymes in hippocampal neurons of APPV717I transgenic mice Chin J Integr Med 2017-06-20 [PMID: 28634862] (WB, Human)

Bioinformatics

• Gene Symbol: MAPT
• Entrez:
  • 4137(Human)