Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein

SDS-Page: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Preformed Fibrils (NBP2-76793). Lane 1: MW Ladder. Lane 2: Tau Protein Preformed Fibrils (NBP2-76793).

In vitro assay: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils (NBP2-76793). Tau Pre-formed Fibrils (NBP2-76793) seed the formation of new tau fibrils when combined with tau monomers . Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

Electron Microscopy: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

Electron Microscopy: Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein [NBP2-76793] - TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.

Product Discontinued

Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein Summary

• Description: An un-tagged partial Human Recombinant Tau Protein Pre-Formed Fibrils, Accession #: P10636
  
  Source: E. coli
  
  Amino Acid Sequence:
  SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
• Details of Functionality: Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MAPT
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • In vitro assay
  • In vivo assay
  • SDS-Page
  • Western Blot
• Theoretical MW: 15.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.4), 100 mM NaCl
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Alternate Names for Recombinant Human Tau Active, (K18), P301L Mutant, Pre-formed Fibrils Protein

• DDPAC
• FLJ31424
• FTDP-17
• G protein beta1/gamma2 subunit-interacting factor 1
• MAPT
• MGC138549
• microtubule-associated protein tau
• MSTD
• MSTDMAPTL
• MTBT1
• MTBT1Neurofibrillary tangle protein
• MTBT2
• Neurofibrillary tangle protein
• PHF-tau
• PPND
• Tau
• TAUPaired helical filament-tau

Background

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MAPT