TCR alpha Antibody (34) - Chimeric - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 34
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TCR alpha Antibody (34) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Purified recombinant soluble TCR from the T-cell hybridoma 85.33, corresponding to V alpha 11
• Specificity: This antibody binds to the T-cell receptor alpha chains.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready. This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. The antibody binds specifically to the recombinant and native variable domain of the alpha chain which comprises the T-cell receptor (TCR). The TCR is a disulfide-linked heterodimeric protein expressed on the surface of T-cells in complex with CD3 and zeta-chain molecules. The receptor functions in the recognition of MHC molecule and the subsequent activation of T-cells. The monoclonal antibody 34 recognises V-alpha regions of TCRs expressed in the 85.33 arthritogenic T-cell hybridoma and can be used to analyse TCR V gene expression and for immunotherapy in T cell mediated autoimmunity.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TCR alpha Antibody (34) - Chimeric - Azide and BSA Free

• C)
• D
• FLJ22602
• J
• MGC117436
• MGC22624
• MGC23964
• MGC71411
• T cell receptor alpha locus
• T-cell antigen receptor, alpha polypeptide
• T-cell receptor, alpha (V
• TCRA
• TCRD
• TRA

Background

T cell receptor (TCR) is a heterodimer consisting of an 945; and a 946; chain (TCR alpha/beta) or a 947; and a delta chain (TCR gamma/delta). TCR-beta is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-alpha). It is expressed on alpah/beta TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The MAB0872 antibody does not cross-react with delta/gamma TCR-bearing T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRA