TCR alpha/beta Antibody (R73) - BSA Free

Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.

Flow Cytometry: TCR alpha/beta Antibody (R73) [NBP2-12941] - Rat thymocytes with anti-rat TCR alpha/beta (R73) PE.

Surface staining pattern of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 ug/ml) GAM APC.

• Reactivity: Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: R73
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TCR alpha/beta Antibody (R73) - BSA Free Summary

• Immunogen: Rat T blasts and erythrocytes
• Specificity: The mouse monoclonal R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-12 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Non-Human Primates.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TCR alpha/beta Antibody (R73) - BSA Free

• C)
• D
• FLJ22602
• J
• MGC117436
• MGC22624
• MGC23964
• MGC71411
• T cell receptor alpha locus
• T-cell antigen receptor, alpha polypeptide
• T-cell receptor, alpha (V
• TCRA
• TCRD
• TRA

Background

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCR alpha/beta Antibody (NBP2-12941)(2)

Publications using NBP2-12941

• Khaldoyanidi S, Schnabel D, Fohr N, Zoller M. Functional activity of CD44 isoforms in haemopoiesis of the rat. Br J Haematol. [PMID: 9012685] (FLOW, Rat)
• Hancock WW, Khoury SJ, Carpenter CB, Sayegh MH. Differential effects of oral versus intrathymic administration of polymorphic major histocompatibility complex class II peptides on mononuclear and endothelial cell activation and cytokine expression during a delayed-type hypersensitivity response. Am J Pathol. [PMID: 8203456] (IHC-Fr, Rat)

Review for TCR alpha/beta Antibody (NBP2-12941) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Xianying Xing IHC-P Human 09/07/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human
• Lot: 528807

Comments

• Comments: I tested on human paraffin embedded tissue and found out that it doesn’t work on human tissue. I used psoriasis lesion tissue and used PH6 and PH9 retrieval buffer but didn’t find any signal. I had a positive control which is a working antibody to make sure the staining system works. The positive control, I used neutrophil elastase(Novus biotechnologies, MAB91671-100) stained on Hidradenitis Suppurativa skin paraffin embedded slides.

Bioinformatics

• Gene Symbol: TRA