TCR beta Antibody (8A3)

Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486] - Analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue).

Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486] - Analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue).

• Reactivity: Hu, Mu, Pm, Ye
• Applications: WB, Flow, IHC, IP
• Clone: 8A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.15 mg/ml

TCR beta Antibody (8A3) Summary

• Immunogen: Human TCR beta chain constant region
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: Tcrb
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2 ug
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 7499875). Mouse reactivity reported in scientific literature (PMID: 21423155). Yeast reactivity reported in scientific literature (PMID: 21159619). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.5% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.15 mg/ml
• Purity: Protein G purified

Alternate Names for TCR beta Antibody (8A3)

• T cell receptor beta chain
• TCRB
• TCRbeta
• Tib

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCR beta Antibody (NBP2-22486)(3)

Publications using NBP2-22486

• He Q, Lu Y, Tian W et al. TOX deficiency facilitates the differentiation of IL-17A-producing gamma sigma T cells to drive autoimmune hepatitis Cellular & molecular immunology 2022-08-19 [PMID: 35986136] (IHC-P)
  
  Details:
  Dilution: 1:200
• Hu Y, Ding J, Wu C et al. Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer Frontiers in Oncology 2022-07-22 [PMID: 35936748] (Human)
• Flores-Villanueva PO, Ganachari M, Guio H et al. An Isolated TCR AB Restricted by HLA-A*02:01/CT37 Peptide Redirecting CD8+T Cells To Kill and Secrete IFN-gamma in Response to Lung Adenocarcinoma Cell Lines. J. Immunol. 2018-03-19 [PMID: 29555781] (Human)

Bioinformatics

• Gene Symbol: Tcrb