TER-119 Antibody (TER-119) [Unconjugated]

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: TER-119
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TER-119 Antibody (TER-119) [Unconjugated] Summary

• Immunogen: C57BL/6 mouse day-14 fetal liver cells
• Specificity: Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly76
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TER-119 Antibody (TER-119) [Unconjugated]

• Ly76
• Ly-76
• TER119
• TER-119

Background

The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TER-119 Antibody (MAB1125)(26)

Publications using MAB1125

• Halder, SK;Sapkota, A;Milner, R; ?1 integrins play a critical role maintaining vascular integrity in the hypoxic spinal cord, particularly in white matter Acta neuropathologica communications 2024-03-20 [PMID: 38509621] (Immunohistochemistry, Mouse)
• Halder, SK;Milner, R; Spinal Cord Blood Vessels in Aged Mice Show Greater Levels of Hypoxia-Induced Vascular Disruption and Microglial Activation International journal of molecular sciences 2023-07-08 [PMID: 37510999] (Immunohistochemistry, Mouse)
• Y Ichiyama, R Matsumoto, S Obata, O Sawada, Y Saishin, M Kakinoki, T Sawada, M Ohji Assessment of mouse VEGF neutralization by ranibizumab and aflibercept PLoS ONE, 2022-12-21;17(12):e0278951. 2022-12-21 [PMID: 36542626] (IHC, Mouse)
• Chisato Watanabe, Hirotoshi Shibuya, Yusuke Ichiyama, Eiichi Okamura, Setsuko Tsukiyama-Fujii, Tomoyuki Tsukiyama et al. Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice Life (Basel) 10/28/2022 [PMID: 36362885]
• Bowen Zhao, Yueqi Ni, Hong Zhang, Yin Zhao, Lu Li Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression Cell Death Discovery 10/28/2022 [PMID: 36307391]
• Laura Maeso-Alonso, Hugo Alonso-Olivares, Nicole Martínez-García, Lorena López-Ferreras, Javier Villoch-Fernández, Laura Puente-Santamaría et al. p73 is required for vessel integrity controlling endothelial junctional dynamics through Angiomotin Cellular and Molecular Life Sciences 10/1/2022 [PMID: 36180740]
• Ando T, Tai-Nagara I, Sugiura Y et al. Tumor-specific inter-endothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness The Journal of clinical investigation 2/1/2022 [PMID: 35104247]
• Ri Yu, Nam-Suk Kim, Yan Li, Jin-Young Jeong, Sang-Joon Park, Bin Zhou et al. Vascular Sema3E-Plexin-D1 Signaling Reactivation Promotes Post-stroke Recovery through VEGF Downregulation in Mice Translational Stroke Research 2/1/2022 [PMID: 33978913]
• I Tai-Nagara, Y Hasumi, D Kusumoto, H Hasumi, K Okabe, T Ando, F Matsuzaki, F Itoh, H Saya, C Liu, W Li, YS Mukouyama, W Marston Li, X Liu, M Hirashima, Y Suzuki, S Funasaki, Y Satou, M Furuya, M Baba, Y Kubota Blood and lymphatic systems are segregated by the FLCN tumor suppressor Nature Communications, 2020-12-09;11(1):6314. 2020-12-09 [PMID: 33298956] (IHC, Mouse)
• A Schlüter, B Aksan, R Diem, R Fairless, D Mauceri VEGFD Protects Retinal Ganglion Cells and, consequently, Capillaries against Excitotoxic Injury Mol Ther Methods Clin Dev, 2019-12-25;17(0):281-299. 2019-12-25 [PMID: 32055648] (IHC, Mouse)
• László Bálint, Zsombor Ocskay, Bálint András Deák, Petra Aradi, Zoltán Jakus Lymph Flow Induces the Postnatal Formation of Mature and Functional Meningeal Lymphatic Vessels Frontiers in Immunology 1/14/2020 [PMID: 31993056]
• G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 2019-11-29;8(0):. 2019-11-29 [PMID: 31782728] (IHC, Mouse)
• R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019-06-27;10(1):2817. 2019-06-27 [PMID: 31249304] (IHC, Mouse)
• Lasse D. Jensen, Belma Hot, Daniel Ramsköld, Raoul F.V. Germano, Chika Yokota, Sarantis Giatrellis et al. Disruption of the Extracellular Matrix Progressively Impairs Central Nervous System Vascular Maturation Downstream of beta -Catenin Signaling Arteriosclerosis, Thrombosis, and Vascular Biology 7/1/2019 [PMID: 31242033]
• Filipa Neto, Alexandra Klaus-Bergmann, Yu Ting Ong, Silvanus Alt, Anne-Clémence Vion, Anna Szymborska et al. YAP and TAZ regulate adherens junction dynamics and endothelial cell distribution during vascular development eLife 2018-02-05 [PMID: 29400648] (Immunohistochemistry, Transgenic Mouse)
• Filipa Neto, Alexandra Klaus-Bergmann, Yu Ting Ong, Silvanus Alt, Anne-Clémence Vion, Anna Szymborska et al. YAP and TAZ regulate adherens junction dynamics and endothelial cell distribution during vascular development eLife 2/5/2018 [PMID: 29400648]
• EM Palmieri, A Menga, R Martín-Pér, A Quinto, C Riera-Domi, G De Tullio, DC Hooper, WH Lamers, B Ghesquière, DW McVicar, A Guarini, M Mazzone, A Castegna Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis Cell Rep, 2017-08-15;20(7):1654-1666. 2017-08-15 [PMID: 28813676] (IHC, Mouse)
• S Li, LR Nih, H Bachman, P Fei, Y Li, E Nam, R Dimatteo, ST Carmichael, TH Barker, T Segura Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability Nat Mater, 2017-08-07;16(9):953-961. 2017-08-07 [PMID: 28783156] (IHC, Mouse)
• I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling Development, 2017-06-02;0(0):. 2017-06-02 [PMID: 28576770] (IHC, Mouse)
• P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al Motor neurons control blood vessel patterning in the developing spinal cord Nat Commun, 2017-03-06;8(0):14583. 2017-03-06 [PMID: 28262664] (IHC, Mouse)
• Blood flow controls bone vascular function and osteogenesis Nat Commun, 2016-12-06;7(0):13601. 2016-12-06 [PMID: 27922003] (IHC, Mouse)
• Haixia Gong, Shejuan An, Antonia Sassmann, Menglin Liu, Victoria Mastej, Manish Mittal et al. PAR1 Scaffolds TGF beta RII to Downregulate TGF-beta Signaling and Activate ESC Differentiation to Endothelial Cells Stem Cell Reports 12/1/2016 [PMID: 27866874]
• Haixia Gong, Shejuan An, Antonia Sassmann, Menglin Liu, Victoria Mastej, Manish Mittal et al. PAR1 Scaffolds TGF beta RII to Downregulate TGF-beta Signaling and Activate ESC Differentiation to Endothelial Cells Stem Cell Reports 2016-12-01 [PMID: 27866874] (Immunohistochemistry, Mouse)
• Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R Integrin beta1 controls VE-cadherin localization and blood vessel stability. Nat Commun, 2015-03-10;6(0):6429. 2015-03-10 [PMID: 25752958] (IHC, Mouse)
• Thomas D. Arnold, Colin Niaudet, Mei-Fong Pang, Julie Siegenthaler, Konstantin Gaengel, Bongnam Jung et al. Excessive vascular sprouting underlies cerebral hemorrhage in mice lacking alpha V beta 8-TGF beta signaling in the brain Development 12/1/2014 [PMID: 25406396]
• Arnold TD, Ferrero GM, Qiu H et al. Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin [alpha]V[beta]8-Mediated Activation of Transforming Growth Factor-[beta]. J Neurosci 2532(4):1197-12. 1/1/2012 [PMID: 22279205]

Review for TER-119 Antibody (MAB1125) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 05/09/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Embryonic spinal cord
• Species: Mouse
• Lot: GRH013041

Bioinformatics

• Gene Symbol: Ly76