TERT Antibody

Western Blot: TERT Antibody [NB110-89471] - Detection of Telomerase reverse transcriptase in HeLa nuclear extracts using NB110-89471.

Immunocytochemistry/Immunofluorescence: TERT Antibody [NB110-89471] - Antibody was tested in V6.5 mouse embryonic stem cells (cat# NBP1-41162) against Dylight 488. Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Product Discontinued

TERT Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human Telomerase reverse transcriptase (within residues 900-950). [Swiss-Prot# O14746]
• Localization: Nuclear
• Marker: Embryonic Stem Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TERT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 0.5 ug/ml
• Application Notes: This Telomerase reverse transcriptase antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~120 kDa. In ICC/IF, puncatate nuclear staining can be seen in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 27876576). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TERT Antibody

• EST2Telomerase-associated protein 2
• hEST2
• hTERT
• TCS1EC 2.7.7.49
• Telomerase catalytic subunit
• telomerase reverse transcriptase
• TERT
• TP2HEST2
• TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TERT Antibody (NB110-89471)(6)

Publications using NB110-89471

• Romaniuk-Drapata A, Toton E, Konieczna N et al. hTERT Downregulation Attenuates Resistance to DOX, Impairs FAK-Mediated Adhesion, and Leads to Autophagy Induction in Breast Cancer Cells Cells 2021-04-10 [PMID: 33920284] (WB, Human)
• Ain Q, Schmeer C, Penndorf D et al. Cell cycle-dependent and -independent telomere shortening accompanies murine brain aging. Aging (Albany, NY) 2018-11-20 [PMID: 30472697] (Simple Western, Mouse)
• Zhang C, Song C, Liu T et al. KMT2A promotes melanoma cell growth by targeting hTERT signaling pathway Cell Death Dis 2017-07-20 [PMID: 28726783] (Human)
• Bugueno J, Li e, Salat P et al. Canine mesenchymal stem cell bone regenerative capacity is regulated by site-specific multi-lineage differentiation. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2016-09-28 [PMID: 27876576] (WB, Canine)
• Walshe J, Harkin DG. Serial explant culture provides novel insights into the potential location and phenotype of corneal endothelial progenitor cells. Exp. Eye Res. 2014-07-14 [PMID: 25035050] (ICC/IF, Human)
  
  Details:
  Telomerase reverse transcriptase antibody used as stem/progenitor cell marker in ICC-IF application on human corneal endothelial cells grown with serial explant culture technique and explant culture wounding model.
• Bandhakavi S, Kim YM, Ro SH et al. Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response. Mol Cell Proteomics 2010-02-01 [PMID: 19955088] (WB, Human)

FAQs for TERT Antibody (NB110-89471). (Showing 1 - 1 of 1 FAQs).

1. Can NB110-89471 be used to do IHC?
   • This product has not been tested for use in IHC however if you would like to test this you will be eligible for our Innovator's Reward Program. Under the terms of this reward we will give you a full credit for testing a product in a previously untested species and/or application regardless of positive or negative results, and submitting the data in the form of a review. Read more about our Innovators Reward Program.

Bioinformatics

• Gene Symbol: TERT
• Entrez:
  • 7015(Human)
• Uniprot:
  • O14746()