TET2 Antibody

Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-27465] - Staining of paraffin embedded Human Spleen. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Flow Cytometry: TET2 Antibody [NBP2-27465] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Ca
• Applications: ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TET2 Antibody Summary

• Immunogen: Peptide with sequence C-PHPQSNNDQQREGSF corresponding to internal region according to NP_001120680.1, NP_060098.3.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001120680.1; NP_060098.3).
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TET2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry 2 - 6 ug/mL
  • Immunohistochemistry-Paraffin 2 - 6 ug/mL
  • Peptide ELISA Detection limit 1:16000
• Application Notes: IHC-P: Human spleen shows nuclear staining in select splenocytes. IHC on human brain sections reported in scientific literature (PMID: 22829908).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TET2 Antibody

• FLJ20032
• KIAA1546
• MGC125715
• Nbla00191
• tet methylcytosine dioxygenase 2
• TET2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TET2 Antibody (NBP2-27465)(1)

Publication using NBP2-27465

• Langemeijer SM, Kuiper RP, Berends M et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nature Gen 2009-07-01 [PMID: 19483684]

Bioinformatics

• Gene Symbol: TET2
• Entrez:
  • 214133(Mouse)