TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (8D3) [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.

Flow Cytometry: TfR (Transferrin R) Antibody (8D3) [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer

• Reactivity: Mu
• Applications: Flow, Func, IHC, CyTOF-ready
• Clone: 8D3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free Summary

• Description: Endotoxin Level <0.01 Eu/ug
• Immunogen: Mouse transformed endothelioma cell line t.end1
• Marker: Recycling Endosome Marker
• Specificity: NB100-64979 recognizes native, soluble and denatured forms of murine CD71. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TFRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:200
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • In vivo assay
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunocytochemistry/Immunofluorescence and In vivo assay were reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% Bovine Serum Albumin
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumor cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anemia and the anemia of chronic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TfR (Transferrin R) Antibody (NB100-64979)(16)

Publications using NB100-64979

• Yu M, Nie Y, Yang J et al. Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease Cell reports 2023-11-28 [PMID: 37925638] (IHC-Fr, Mouse)
• Liu G, Wang J, Wei Z et al. Elevated PDGF-BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFR? Shedding from Pericytes Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-04-27 [PMID: 37102631] (IHC-Fr, Mouse)
• Palhol JSC, Balia M, Sánchez-Román Terán F et al. Direct association with the vascular basement membrane is a frequent feature of myelinating oligodendrocytes in the neocortex Fluids and barriers of the CNS 2023-04-03 [PMID: 37013659] (IHC, Mouse)
• Kuo A, Checa A, Niaudet C et al. Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasis eLife 2022-10-05 [PMID: 36197001] (Immunohistochemistry, Mouse)
• Masuda T, Amann L, Monaco G et al. Specification of CNS macrophage subsets occurs postnatally in defined niches Nature 2022-04-01 [PMID: 35444273] (IHC-Fr, Mouse)
• Li D, Peng J, Li T et al. Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia Molecular medicine reports 2021-06-01 [PMID: 33880575]
• Yang AC, Stevens MY, Chen MB et al. Physiological blood-brain transport is impaired with age by a shift in transcytosis Nature 2020-07-01 [PMID: 32612231] (IF/IHC, Mouse)
• Meister SW, Hjelm LC, Dannemeyer M et al. An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor Int J Mol Sci 2020-04-23 [PMID: 32340383] (FLOW)
• Yanagida K, Engelbrecht E, Niaudet C et al. Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization Dev. Cell 2020-03-23 [PMID: 32059774]
• DarYadel A, Bettoni C, Haider T et al. Erythropoietin stimulates fibroblast growth factor 23 (FGF23) in mice and men. Pflugers Arch. 2018-07-02 [PMID: 29961920] (IF/IHC, Mouse)
• Michael Vanlandewijck, Liqun He, Maarja Andaloussi Mäe, Johanna Andrae, Koji Ando, Francesca Del Gaudio, Khayrun Nahar, Thibaud Lebouvier, Bàrbara Laviña, Leonor Gouveia, Ying Sun, Elisabeth Raschperger, Markus Räsänen, Yvette Zarb, Naoki Mochizuki, Annika Keller, Urban Lendahl, Christer Betsholtz A molecular atlas of cell types and zonation in the brain vasculature. Nature 2018-07-09 [PMID: 29443965]
• Papademetriou IT, Garnacho C, Schuchman EH, Muro S. In vivo performance of polymer nanocarriers dually-targeted to epitopes of the same or different receptors. Biomaterials 2013-02-08 [PMID: 23398883] (In Vivo, Mouse)
• Papademetriou J, Garnacho C, Serrano D et al. Comparative binding, endocytosis, and biodistribution of antibodies and antibody-coated carriers for targeted delivery of lysosomal enzymes to ICAM-1 versus transferrin receptor J Inherit Metab Dis 2012-09-12 [PMID: 22968581] (In Vivo, Mouse)
• Ruderisch N, Virgintino D, Makrides V, Verrey F. Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3 J Cereb Blood Flow Metab 2011-07-01 [PMID: 21364602] (ICC/IF, IF/IHC, Mouse)
• Lee, HJ et al. Targeting rat anti-mouse transferin receptor monoclonal antibodies through blood-brain barrier in mouse. J Pharmacol Exp Ther 292: 1048-52. 2000-01-01 [PMID: 10688622]
• Kissel, K et al. Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibody. Histochem cell Biol 110: 63-72. 1998-01-01 [PMID: 9681691]

Review for TfR (Transferrin R) Antibody (NB100-64979) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Inês Ribeiro ICC Mouse 01/14/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: bEnd.3 mouse endothelioma cell line
• Species: Mouse
• Lot: 150030

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** 0.1 % triton BSA 30 min Primary Ab: 1:50, 1h, RT Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min

Bioinformatics

• Gene Symbol: TFRC
• Uniprot:
  • Q62351()