TGF-beta 1 Antibody

Western Blot: TGF-beta 1 Antibody [NBP1-45891] - Mouse 3T3 cell lysate with TGF-beta 1 antibody.

Immunocytochemistry/Immunofluorescence: TGF-beta 1 Antibody [NBP1-45891] - Co-localization of F4/80+ and TGF-B1 at tendon injury site 1 week after burn/tenotomy. Scale bars correspond to 100um. d Left: Co-localization of CD68+ and TGF-B1 in early human HO. Right: Co-localization of p-SMAD2 and PDGFRa in human HO. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-14172-4), licensed under a CC-BY license.

Product Discontinued

TGF-beta 1 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab92486)
• Immunogen: Synthetic peptide surrounding amino acid 374 of human TGF beta 1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 0.5 - 4 ug/mL
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32024825).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for TGF-beta 1 Antibody

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

Transforming growth factor betas (TGFbetas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFalpha. It is now realized that TGFbetas mediate many cell-cell interactions that occur during embryonic development. Three TGFbetas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TGF-beta 1 Antibody (NBP1-45891)(7)

Publications using NBP1-45891

• Chen W, Pilling D, Gomer RH The mRNA binding protein DDX3 mediates TGF-?1 upregulation of translation and promotes pulmonary fibrosis JCI insight 2023-02-23 [PMID: 36821384] (IHC-Fr, Mouse)
• Jin M, Lee J, Lee Ky et al. Alteration of TGF-beta-ALK-Smad signaling in hyperoxia-induced bronchopulmonary dysplasia model of newborn rats. Exp. Lung Res. 2016-09-12 [PMID: 27618520] (WB)
• Wang Y, Huang X, Tang Y et al. Effects of panax notoginseng saponins on the osteogenic differentiation of rabbit bone mesenchymal stem cells through TGF-beta1 signaling pathway. BMC Complement Altern Med. 2016-08-26 [PMID: 27561678] (WB)
• Huber AK, Sorkin M, Hwang C, et al. Regulation of heterotopic ossification through local inflammatory monocytes in a mouse model of aberrant wound healing bioRxiv (ICC/IF, Mouse)
• Jo YH, Lee H, Oh MH et al. Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model Nutr Res Pract 2020-08-09 [PMID: 32765814]
• Park MH, Kwon SY, Choi JE et al. Intratumoral CD103-positive tumor-infiltrating lymphocytes are associated with favorable prognosis in patients with triple-negative breast cancer Histopathology 2020-04-25 [PMID: 32333690]
• Bourdon R Regulation of epinephrine biosynthesis by intermittent and continuous hypoxia Nat Commun 2020-02-07 [PMID: 32024825] (ICC/IF, Mouse)

Review for TGF-beta 1 Antibody (NBP1-45891) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Charles Hwang IHC-P Mouse 07/11/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse tissue
• Species: Mouse
• Lot: 1G15559

Comments

• Comments: Paraffin sections
  Citrate 6.0 HIER 30min + 20min cooled at RT
  AF488 and AF594

Bioinformatics

• Gene Symbol: TGFB1
• Entrez:
  • 7040(Human)
• Uniprot:
  • P01137(Human)