Canine TGF-beta 1 ELISA Kit (Colorimetric)

ELISA: Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147] - Standard Curve Reference

• Reactivity: Ca
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 0.781 ng/mL - 50 ng/mL
• Sensitivity: 0.747 ng/mL

Canine TGF-beta 1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of dog TGF-B1. No significant cross-reactivity or interference between dog TGF-B1 and analogues was observed.
• Standard Curve Range: 0.781 ng/mL - 50 ng/mL
• Sensitivity: 0.747 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TGFB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Canine TGF-beta 1 ELISA Kit (Colorimetric)

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TGFB1