Recombinant TGF-beta 3 Protein

Product Discontinued

Recombinant TGF-beta 3 Protein Summary

• Description: A biologically reactive recombinant protein of Human TGF-beta 3.
  
  

  Source: Nicotiana benthamiana

  Amino Acid Sequence: HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

• Epitope: HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
• Protein/Peptide Type: Recombinant Protein
• Gene: TGFB3

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
  • Western Blot
• Application Notes: Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Tris HCl 0.05M buffer at pH 7.4.
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute with water to a final concentration of 50 ng/ul. Due to the protein nature, dimmers and multimers may be observed.

Notes

Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ìg protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

Alternate Names for Recombinant TGF-beta 3 Protein

• ARVD
• ARVD1
• FLJ16571
• LDS5
• RNHF
• TGFB3
• TGFbeta 3
• TGF-beta 3
• TGF-beta3
• TGF-beta-3
• transforming growth factor beta-3
• transforming growth factor, beta 3

Background

Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TGFB3