Recombinant Human TGF-beta 3 Animal-Free Protein

SDS-Page: Recombinant Human TGF-beta 3 Animal-Free Protein [NBP2-76236] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human TGF-beta 3 Animal-Free Protein [NBP2-76236] - TGF-beta 3 inhibition of IL-4 induced HT-2 cell proliferation.

Product Discontinued

Recombinant Human TGF-beta 3 Animal-Free Protein Summary

• Description: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
  
  Expiration Date:
  12 months from date of receipt when stored at 4C as supplied.
• Specificity: TGF-beta 3 is a dimer, 12.9/25.7 kDa (113/226 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: Inhibition of IL-4-induced HT-2 cell proliferation, >1.0 x 10^6 units/mg (ED50 acceptance criteria are <1 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TGFB3
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM acetic acid and 20% Ethanol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human TGF-beta 3 Animal-Free Protein

• ARVD
• ARVD1
• FLJ16571
• LDS5
• RNHF
• TGFB3
• TGFbeta 3
• TGF-beta 3
• TGF-beta3
• TGF-beta-3
• transforming growth factor beta-3
• transforming growth factor, beta 3

Background

Transforming Growth Factor beta 3 (TGF beta 3) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional, capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TGFB3