TGF-beta Antibody (1D11.16.8) - Azide and BSA Free

Immunohistochemistry: TGF-beta Antibody (1D11.16.8) - Azide and BSA Free [NBP2-47736] - TGF-beta was detected in immersion fixed paraffin-embedded human skin using 25 ug/mL Mouse Anti-TGF-beta Monoclonal Antibody (Clone 1D11.16.8) overnight at 4 degrees C. Tissues was stained using the Anti-Mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with Haematoxylin (blue).

Immunohistochemistry: TGF-beta Antibody (1D11.16.8) - Azide and BSA Free [NBP2-47736] - TGF-beta was detected in immersion fixed paraffin-embedded human skin using 25 ug/mL Mouse Anti-TGF-beta Monoclonal Antibody (Clone 1D11.16.8) overnight at 4 degrees C. Tissues was stained using the Anti-Mouse HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with Haematoxylin (blue).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ha, Pm
• Applications: ELISA, Func, ICC/IF, IHC, B/N, ELISA(Cap), Inhibition Activity
• Clone: 1D11.16.8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TGF-beta Antibody (1D11.16.8) - Azide and BSA Free Summary

• Immunogen: Bovine bone-derived TGF-beta 1 and TGF-beta 2
• Localization: Cytoplasm and extracellular (secreted)
• Specificity: This MAb recognizes TGF beta 1, 2 and 3. Three TGF betas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. TGFbeta's inhibit the growth of epithelial cells and stimulate the growth of mesenchymal cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • Functional
  • Immunocytochemistry/ Immunofluorescence 8-25 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0ug/ml
  • Inhibition Activity
  • Neutralization
  • Sandwich ELISA
• Application Notes: Hu-chromosome location: 19q13.1 (beta1); 1q41 (beta2); 14q24 (beta3). Order Ab without BSA/Azide for Functional Assay and Cytokine Neutralization
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for TGF-beta Antibody (1D11.16.8) - Azide and BSA Free

• CED
• DPD1
• LAP
• TGFB
• TGFB1
• TGFbeta
• TGF-beta
• transforming growth factor beta 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFB1