TGN46 Antibody (2F11) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

ELISA: TGN46 Antibody (2F11) [H00010618-M02] - Detection limit for recombinant GST tagged TGOLN2 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TGN46 Antibody (2F11) - Azide and BSA Free Summary

• Immunogen: TGOLN2 (NP_006455, 229 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QGPIDGPSKSGAEEQTSKDSPNKVVPEQPSRKDHSKPISNPSDNKELPKADTNQLADKGKLSPHAFKTESGEETDLISPPQEEVKSSEPTEDVEPKEAE
• Marker: TGN Marker
• Specificity: TOGLN2 (2F11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGOLN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TGN46 Antibody (2F11) - Azide and BSA Free

• TGN 46
• TGN 48
• TGN 51
• TGN38 homolog
• TGN38
• TGN-38
• TGN46
• TGN-46
• TGN48
• TGN51
• TGOLN 2
• TGOLN2
• trans golgi network 38
• trans golgi network 46
• Trans Golgi network integral membrane protein 2 [Precursor]
• Trans Golgi network integral membrane protein 2
• Trans Golgi network protein (46 48 51kD isoforms)
• Trans golgi network protein 2
• Trans Golgi network protein TGN51
• Trans-Golgi network integral membrane protein 2
• Trans-Golgi network protein 2
• Trans-Golgi network protein TGN51
• TTGN 2
• TTGN2

Background

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are exist, TGN46, TGN48 and TGN51. Isoform TGN46 is the more widely expressed isoform. TGN48 is expressed in embryonic kidney and promyelocytic cells. Isoform TGN51 is abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (H00010618-M02)(7)

Publications using H00010618-M02

• Charles AS, Chouljenko VN, Jambunathan N et al. Phenylalanine Residues at the Carboxyl-terminus of the Herpes Simplex Virus Type-1 UL20 Membrane Protein Regulate Cytoplasmic Virion Envelopment and Infectious Virus Production. J Virol. 2014-04-23 [PMID: 24760889]
• Yoshida K, Asakawa M, Suzuki-Kouyama E et al. Distinctive features of degenerating Purkinje cells in spinocerebellar ataxia type 31. Neuropathology. 2013-12-17 [PMID: 24344778]
• Higashi S, Iseki E, Minegishi M et al. GIGYF2 is present in endosomal compartments in the mammalian brains and enhances IGF-1-induced ERK1/2 activation. J Neurochem. 2010-07-28 [PMID: 20670374]
• Lee J, Hwang H, Kang D et al. Therapy-induced senescent cancer cells contribute to cancer recurrence by providing a PD-L1 umbrella regulated by ribophorin 1 Research Square 2023-10-09
• Barr AR, Kilmartin JV, Gergely F et al. CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response. J Cell Biol 2010-04-01 [PMID: 20368616]
• Higashi S, Moore DJ, Yamamoto R et al. Abnormal localization of leucine-rich repeat kinase 2 to the endosomal-lysosomal compartment in lewy body disease. J Neuropathol Exp Neurol 68(9):994-1005. 2009-09-01 [PMID: 19680143]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
• OMIM:
  • 603062(Human)
• Uniprot:
  • NP_006455(Human)