| Description | Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.
If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label: 1. Protein Thiolation Kit Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein. Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working. 2. Thiol Quantification Kit The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction. The assay is in a convenient 96-well plate format and contains all the necessary reagents required. |
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| Kit Type | Quantification Kit |
| Application Notes | Lightning-Link technology can also be used to label proteins, peptides and other biomolecules (See protocol for further information). |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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