Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein

SDS-Page: Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein [NBP1-44456] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 161-647 of Human Thioredoxin Reductase 1/TRXR1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC

• Details of Functionality: Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TXNRD1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 55.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Thioredoxin Reductase 1/TRXR1 His Protein

• EC 1.8.1
• EC 1.8.1.9
• Gene associated with retinoic and IFN-induced mortality 12 protein
• Gene associated with retinoic and interferon-induced mortality 12 protein
• GRIM12
• GRIM-12
• GRIM-12TR1
• KDRF
• KM-102-derived reductase-like factor
• MGC9145
• oxidoreductase
• Thioredoxin Reductase 1
• thioredoxin reductase 1, cytoplasmic
• thioredoxin reductase GRIM-12
• Thioredoxin reductase TR1
• TR
• TR1
• TRXR1
• TRXRD1
• TXNR
• TXNRD1
• TXNRTRXR1

Background

TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Thioredoxin Reductase 1/TRXR1 Recombinant Protein (NBP1-44456)(1)

Publication using NBP1-44456

• Beyrath J, Pellegrini M, Renkema H et al. KH176 Safeguards Mitochondrial Diseased Cells from Redox Stress-Induced Cell Death by Interacting with the Thioredoxin System/Peroxiredoxin Enzyme Machinery Sci Rep 2018-04-26 [PMID: 29700325] (BA, Human)

FAQs for Thioredoxin Reductase 1/TRXR1 Recombinant Protein (NBP1-44456). (Showing 1 - 1 of 1 FAQ).

1. Does this protein contain selenocystein?
   • According to the lab, the selenocysteine was cleaved for this protein. The domain of our recombinant human thioredoxin reductase 1 is 161-647aa. which corresponds to the TGR domain (161-649aa) minus the selenocysteine.

Bioinformatics

• Gene Symbol: TXNRD1