Recombinant Mouse Thrombopoietin/THPO Protein

SDS-Page: Recombinant Mouse Thrombopoietin/THPO Protein [NBP2-35186]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse Thrombopoietin/THPO Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to Thrombopoietin/THPO Source: E. coli

  Uniprot ID: P40226

  Amino Acid Sequence: SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

• Details of Functionality: Thrombopoietin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: THPO
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Thrombopoietin/THPO as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 18.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose.
• Preservative: 5% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse Thrombopoietin/THPO Protein

• Megakaryocyte colony-stimulating factor
• Megakaryocyte growth and development factor
• megakaryocyte stimulating factor
• MGDF
• MGDFC-mpl ligand
• MKCSF
• MK-CSF
• ML
• MPL ligand
• MPLLG
• MPLLGMGC163194
• Myeloproliferative leukemia virus oncogene ligand
• THCYT1
• THPO
• thrombopoietin nirs variant 1
• Thrombopoietin
• Tpo
• TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. Tpo additionally promotes platelet aggregation, ECM adhesion, and activation. In the brain, Tpo promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: THPO
• Uniprot:
  • P40226()
  • P40226()
  • P40226()