Thrombospondin-1 Antibody (A65M) - BSA Free

• Reactivity: Hu, Bv
• Applications: ELISA, IHC, IP
• Clone: A65M
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Thrombospondin-1 Antibody (A65M) - BSA Free Summary

• Immunogen: Extracellular matrix material from cultured bovine corneal endothelial cells
• Epitope: Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
• Specificity: Highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: THBS1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: IHC: NBP1-05185 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. NBP1-05185 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Thrombospondin-1 Antibody (A65M) - BSA Free

• THBS
• THBS1
• THBS-1
• thrombospondin 1
• Thrombospondin1
• Thrombospondin-1
• thrombospondin-1p180
• TSP-1
• TSP1thrombospondin-1
• TSPTSP-1

Background

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thrombospondin-1 Antibody (NBP1-05185)(1)

Publication using NBP1-05185

• Matthias LJ, Gotis-Graham I, Underwood PA et al. Identification of monoclonal antibodies that recognize different disulfide bonded forms of thrombospondin 1. Biochim Biophys Acta. 1996-09-01 [PMID: 8814219]

Bioinformatics

• Gene Symbol: THBS1