Thymosin alpha 1 Antibody

Immunohistochemistry-Paraffin: Thymosin alpha 1 Antibody [NBP1-36979] - (3.75ug/ml) staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Thymosin alpha 1 Antibody Summary

• Immunogen: Peptide with sequence C-DVDTKKQKTDEDD corresponding to C-Terminus according to NP_001092755.1, NP_002814.3.
• Epitope: C Terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001092755.1; NP_002814.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTMA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:1000
• Application Notes: WB: Preliminary experiments gave an approx. 38 kDa band in lysates of A431 after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.2 kDa band according to NP_001092755.1 and of 12.1 kDa band according to NP_002814.3. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.

Reactivity Notes

Expected from sequence similarity: Rat, Dog

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Thymosin alpha 1 Antibody

• alpha (gene sequence 28)
• gene sequence 28
• MGC104802
• prothymosin alpha protein
• prothymosin alpha
• prothymosin, alpha
• PTMA prothymosin, alpha
• TMSA

Background

Thymosin alpha 1 is a protein that helps mediate immune function by conferring resistance to various infections, and has two isoforms, with lengths of 111 and 110 amino acids and both weighing approximately 12 kDa. Current studies are being done on several diseases and disorders related to this protein including severe acute respiratory syndrome, myasthenia gravis, chronic obstructive pulmonary disease, hepatitis, liver cirrhosis, male infertility, thymoma, pituitary adenoma, hepatocellular carcinoma, hepatoblastoma, sepsis, tonsillitis, gastric adenocarcinoma, and breast cancer. Thymosin alpha 1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the C-MYC transcriptional activation pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thymosin alpha 1 Antibody (NBP1-36979)(1)

Publication using NBP1-36979

• Uren AG, Kool J, Matentzoglu K et al. Large-scale mutagenesis in p19(ARF)- and p53-deficient mice identifies cancer genes and their collaborative networks. Cell 2008-05-01 [PMID: 18485879]

Bioinformatics

• Gene Symbol: PTMA
• Entrez:
  • 5757(Human)
  • 500448(Rat)
• Uniprot:
  • NP_001092755.1(Human)